Cloning and expression of the rat prolactin receptor, a member of the growth hormone/prolactin receptor gene family

• Published in: Cell Vol. 53; no. 1; p. 69
• Main Authors: Boutin, J M, Jolicoeur, C, Okamura, H, Gagnon, J, Edery, M, Shirota, M, Banville, D, Dusanter-Fourt, I, Djiane, J, Kelly, P A
• Format: Journal Article
• Language: English
• Published: United States 08-04-1988
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA - genetics; DNA Restriction Enzymes; Female; Genes; Liver - metabolism; Microsomes, Liver - metabolism; Molecular Sequence Data; Oocytes - metabolism; Protein Biosynthesis; Rats; Receptors, Prolactin - genetics; Receptors, Prolactin - metabolism; Receptors, Somatotropin - genetics; Transcription, Genetic; Xenopus laevis
• ISSN: 0092-8674
• DOI: 10.1016/0092-8674(88)90488-6

The primary structure of the rat liver prolactin receptor has been deduced from a single complementary DNA clone. The sequence begins with a putative 19 amino acid signal peptide followed by the 291 amino acid receptor that includes a single 24 amino acid transmembrane segment. In spite of the fact that the prolactin receptor has a much shorter cytoplasmic region than the growth hormone receptor, there is strong localized sequence identity between these two receptors in both the extracellular and cytoplasmic domains, suggesting that the two receptors originated from a common ancestor.