Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection

• Published in: Journal of general virology Vol. 91; no. 1; pp. 294 - 305
• Main Authors: Satoh, Kouji, Kondoh, Hiroaki, Sasaya, Takahide, Shimizu, Takumi, Choi, Il-Ryong, Omura, Toshihiro, Kikuchi, Shoshi
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-01-2010; Society for General Microbiology
• Subjects: Biological and medical sciences; cytopathogenicity; disease reservoirs; Down-Regulation; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; Gene Expression Regulation; Genes, Plant; Genetics; host-pathogen relationships; infection; microarray technology; Microbiology; Miscellaneous; Oligonucleotide Array Sequence Analysis; Oryza - physiology; Oryza - virology; Oryza sativa; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant viruses and viroids; resistance mechanisms; rice; Rice stripe virus; Tenuivirus - pathogenicity; transcription (genetics); transcriptome; Up-Regulation; Virology; Virus; Infection; Rice stripe virus; Oryza sativa; Monocotyledones; Plant pathogen; Gramineae; Angiospermae; Spermatophyta; Tenuivirus; Gene expression; Rice
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/vir.0.015990-0

Rice stripe disease, caused by rice stripe virus (RSV), is one of the major virus diseases in east Asia. Rice plants infected with RSV usually show symptoms such as chlorosis, weakness, necrosis in newly emerged leaves and stunting. To reveal rice cellular systems influenced by RSV infection, temporal changes in the transcriptome of RSV-infected plants were monitored by a customized rice oligoarray system. The transcriptome changes in RSV-infected plants indicated that protein-synthesis machineries and energy production in the mitochondrion were activated by RSV infection, whereas energy production in the chloroplast and synthesis of cell-structure components were suppressed. The transcription of genes related to host-defence systems under hormone signals and those for gene silencing were not activated at the early infection phase. Together with concurrent observation of virus concentration and symptom development, such transcriptome changes in RSV-infected plants suggest that different sets of various host genes are regulated depending on the development of disease symptoms and the accumulation of RSV.