New Experimental Model of Acute Renal Failure and Sepsis in Rats

• Published in: JPEN. Journal of parenteral and enteral nutrition Vol. 18; no. 6; pp. 477 - 485
• Main Authors: Kuhlmann, Martin K., Shahmir, Ehsan, Maasarani, Essam, Akhtar, Salman, Thevanayagam, Vasuki, Vadgama, Jaydutt V., Kopple, Joel D.
• Format: Journal Article
• Language: English
• Published: Thousand Oaks, CA Sage Publications 01-11-1994; SAGE Publications; ASPEN
• Subjects: Acute Kidney Injury - complications; Acute Kidney Injury - physiopathology; Amino Acids - blood; Ammonia - blood; Animals; Biological and medical sciences; Cecum; Constriction; Disease Models, Animal; Ligation; Male; Medical sciences; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Nitrogen - blood; Punctures; Rats; Rats, Sprague-Dawley; Renal Artery; Renal failure; Sepsis - complications; Sepsis - physiopathology; Sodium Bicarbonate - pharmacology; Urea - blood; Infection; Animal model; Vertebrata; Urinary system disease; Mammalia; Nephropathy; Rat; Animal; Rodentia; Renal failure; Renal disease; Catabolism
• ISSN: 0148-6071; 1941-2444
• DOI: 10.1177/0148607194018006477

Background: A rat model of acute renal failure (ARF) with sepsis (ARF+S) was developed to simulate the clinical syndrome of hypercatabolic illness in patients with ARF. Methods: Sepsis was created by ligation and needle puncture of the cecum; ARF was created by left renal artery clamping and contralateral nephrectomy. Results: Two studies were performed. In study 1, rats with sham surgery, sepsis, ARF, and ARF+S were examined for 48 hours. During the first 24 hours after surgery, ARF and ARF+S rats displayed increased urea and ammonia nitrogen appearances and abnormal plasma amino acid levels. These abnormalities were exaggerated in ARF+S rats. In study 2, sham, ARF, and ARF+S rats were injected with sodium bicarbonate or normal saline. During the first 24 hours after surgery, the ARF and ARF+S rats showed an increase in urea nitrogen appearance to 210% and 293%, respectively, of sham values, which was greater than the levels that have been previously reported. Sodium bicarbonate treatment did not influence nitrogen output. Conclusions: Rats with ARF+S may be a useful model for studying catabolic patients with ARF. The lack of effect of sodium bicarbonate on nitrogen balance merits additional study. (Journal of Parenteral and Enteral Nutrition
18:477-485, 1994)