High expression of CDCA7 predicts tumor progression and poor prognosis in human colorectal cancer

• Published in: Molecular medicine reports Vol. 22; no. 1; pp. 57 - 66
• Main Authors: Li, Siman, Huang, Jiean, Qin, Mengbin, Zhang, Jinxiu, Liao, Cun
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01-07-2020; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Adult; Age; Aged; Analysis; Antibodies; Bioinformatics; Cancer; Cancer metastasis; Cancer research; Cell cycle; Cell division; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Development and progression; Disease; Disease Progression; DNA methylation; Female; Gene expression; Gene Expression Regulation, Neoplastic; Genomes; Humans; Immunohistochemistry; Lymph nodes; Lymphatic Metastasis - diagnosis; Lymphatic Metastasis - genetics; Lymphatic Metastasis - pathology; Male; Medical prognosis; Metastases; Metastasis; MicroRNAs; Middle Aged; Neoplasm Invasiveness - diagnosis; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; Nuclear Proteins - analysis; Nuclear Proteins - genetics; Prognosis; Proteins; Researchers; Reverse transcription; Scientific equipment industry; Transcription factors; Tumors; Up-Regulation; Western blotting; United States; cell division cycle associated protein 7; progression; colorectal cancer; prognosis; bioinformatics analysis
• DOI: 10.3892/mmr.2020.11089
• EISSN: 1791-3004
• ISSN: 1791-2997; 1791-3004

Colorectal cancer (CRC) is one of the most fatal types of cancer worldwide. This study aimed to determine the predictive and prognostic values of cell division cycle associated protein 7 (CDCA7) in CRC. Firstly, the relationship between CDCA7 and CRC was assessed through bioinformatics analysis. Subsequently, CDCA7 expression levels were detected in various CRC cell lines, as well as 15 fresh human CRC tissues and their paired adjacent normal colorectal tissues using reverse transcription‑quantitative PCR and western blotting. Additionally, immunohistochemical staining was used to determine the levels of CDCA7 in 104 CRC tissues and their paired adjacent normal colorectal tissues. The present study revealed that CDCA7 expression was upregulated in CRC tissues and cell lines. The positive expression rates of CDCA7 in normal and CRC tissues were 26.92 and 75.96%, respectively. The intensities of CDCA7 immunostaining were significantly associated with CRC invasion depth, lymph node metastasis, tumor‑node‑metastasis stage and distant metastasis. However, no significant differences in sex, age, tumor size and CRC differentiation were found between high and low CDCA7 expression groups. Furthermore, patients with low CDCA7 expression exhibited a greater overall survival rate of CRC compared to those with high CDCA7 expression. The findings of this study indicated that CDCA7 may serve a significant role in CRC prognosis and progression, and may be considered a novel biomarker for the prediction of patient survival after colectomy.