Development and Application of a Cleaved Amplified Polymorphic Sequence Marker ( Phyto ) Linked to the Pc5.1 Locus Conferring Resistance to Phytophthora capsici in Pepper ( Capsicum annuum L.)

• Published in: Plants (Basel) Vol. 12; no. 15; p. 2757
• Main Authors: Bongiorno, Giacomo, Di Noia, Annamaria, Ciancaleoni, Simona, Marconi, Gianpiero, Cassibba, Vincenzo, Albertini, Emidio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25-07-2023; MDPI
• Subjects: Biomarkers; CAPS markers; Capsicum annuum; Chromosomes; Crop diseases; Cultivars; Gene sequencing; Genes; Genomes; Genotypes; Germplasm; Homoserine kinase; Horticulture; Kinases; Loci; Marker-assisted selection; Nucleotide sequence; Pathogens; Peppers; Phenotypes; Phytochemicals; Phytophthora capsici; Quantitative trait loci; Single-nucleotide polymorphism; soil-borne pathogens; Virulence; Capsicum annuum; marker-assisted selection; soil-borne pathogens; CAPS markers; Phytophthora capsici
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12152757

causes destructive disease in several crop species, including pepper (
L.). Resistance in this species is physiologically and genetically complex due to many
virulence phenotypes and different QTLs and R genes among the identified resistance sources. Several primer pairs were designed to follow an SNP (G/A) within the
locus linked to the
locus. All primer pairs were designed on DNA sequences derived from
, a homoserine kinase (HSK), which is a gene candidate responsible for the major QTL on chromosome
for resistance to
. A panel of 69 pepper genotypes from the Southern Seed germplasm collection was used to screen the primer pairs designed. Of these, two primers (
_for_2 and
_rev_2) surrounding the SNP proved successful in discriminating susceptible and resistant genotypes when combined with a restriction enzyme (
). This new marker (called
) worked as expected in all genotypes tested, proving to be an excellent candidate for marker-assisted selection in breeding programs aimed at introgressing the resistant locus into pure lines.