Cloning, sequence analysis and three-dimensional structure prediction of DNA pol I from thermophilic Geobacillus sp. MKK isolated from an Iranian hot spring

Molecular phylogenetic analysis of a novel thermophilic eubacterium isolated from an Iranian hot spring using 16S rDNA sequence showed that the new isolate belongs to genera Geobacillus. DNA pol I gene from this isolate was amplified, cloned, sequenced, and the three-dimensional (3D) structure of de...

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Bibliographic Details
Published in:	Applied biochemistry and biotechnology Vol. 142; no. 2; pp. 200 - 208
Main Authors:	KHALAJ-KONDORI, Mohammad, SADEGHIZADEH, Majid, KHAJEH, Khosro, NADERI-MANESH, Hossein, ALI MOHAMMAD AHADI, EMAMZADEH, Abdorahman
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-08-2007 Springer Nature B.V
Subjects:	Amino Acid Sequence Amino acids Bacillaceae - classification Bacillaceae - enzymology Bacillus stearothermophilus Bacteria Binding sites Biological and medical sciences Biotechnology Cloning Cloning, Molecular Deoxyribonucleic acid DNA DNA Polymerase I - chemistry DNA Polymerase I - genetics DNA, Ribosomal - chemistry Enzymes Eubacterium Exonucleases - chemistry Fundamental and applied biological sciences. Psychology Geobacillus Hot springs Hot Springs - microbiology Iran Models, Molecular Molecular Sequence Data Phylogeny Protein Structure, Tertiary Sequence Analysis Iran Thermostable DNA pol I Three dimensional structure Gsm pol I DNA Cloning Prediction Thermophily Hot source Phylogeny Thermal stability 3D structure prediction Molecular phylogenetic
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