Long-term effects of repetitive exposure to a static magnetic field (1.5 T) on proliferation of human fetal lung fibroblasts

• Published in: Magnetic resonance in medicine Vol. 41; no. 3; pp. 464 - 468
• Main Authors: Wiskirchen, J., Groenewaeller, E.F., Kehlbach, R., Heinzelmann, F., Wittau, M., Rodemann, H.P., Claussen, C.D., Duda, S.H.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01-03-1999; Williams & Wilkins
• Subjects: Biological and medical sciences; biological effects; Cell Division - radiation effects; Cell physiology; Cells, Cultured - radiation effects; Effects of physical and chemical agents; Electromagnetic Fields - adverse effects; experimental; Fetus - radiation effects; Fibroblasts - cytology; Fibroblasts - radiation effects; Fundamental and applied biological sciences. Psychology; Humans; Lung - anatomy & histology; Lung - cytology; Lung - embryology; magnetic resonance (MR); Magnetic Resonance Imaging - adverse effects; Magnetic Resonance Imaging - methods; Molecular and cellular biology; MR, biological effects; MR, experimental; MR, safety; Reference Values; safety; Statistics, Nonparametric; Time Factors; Human; Cell proliferation; Static field; Lung; Magnetic resonance; Fetus; Biological effect; Respiratory system; Magnetic field; Fibroblast; Long term
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/(SICI)1522-2594(199903)41:3<464::AID-MRM6>3.0.CO;2-R

The aim of the study was to assess the effects of repetitive exposures to a static magnetic field (1.5 T) on human fetal lung fibroblast (HFL) proliferation. HFL were exposed three times a week for 1 hr to a static magnetic field for 3 weeks. Cells were subcultured every week. Population doublings (PD) and cumulative population doublings (CPD) were calculated weekly. Colony formation assays, bromodeoxyuridine enzyme‐linked immunosorbent assay, and cell cycle analysis were performed weekly. After the third week, proliferation kinetics were assessed. Over a period of 3 weeks no statistically significant differences between the PD and CPD of exposed and control cells could be detected. Clonogenic activity, DNA synthesis, cell cycle, and proliferation kinetics were not altered by magnetic field exposure. The data do not provide evidence that repetitive exposures to a static magnetic field (1.5 T) exert effects on HFL proliferation. Magn Reson Med 41:464–468, 1999.  © 1999 Wiley‐Liss, Inc.