Analysis of a diverse assemblage of diazotrophic bacteria from Spartina alterniflora using DGGE and clone library screening

Methods to assess the diversity of the diazotroph assemblage in the rhizosphere of the salt marsh cordgrass, Spartina alterniflora were examined. The effectiveness of nifH PCR-denaturing gradient gel electrophoresis (DGGE) was compared to that of nifH clone library analysis. Seventeen DGGE gel bands...

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Bibliographic Details
Published in:	Journal of microbiological methods Vol. 73; no. 2; pp. 160 - 171
Main Authors:	Lovell, Charles R., Decker, Peter V., Bagwell, Christopher E., Thompson, Shelly, Matsui, George Y.
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier B.V 01-05-2008 Elsevier Science
Subjects:	Assemblage composition Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacterial Proteins - genetics Bacteriology Biodiversity Biological and medical sciences Clone library Cluster Analysis DGGE Diazotroph DNA Primers - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Microbiology Miscellaneous Molecular Sequence Data Oxidoreductases - genetics Phylogeny Plant Roots - microbiology Poaceae - microbiology Sequence Analysis, DNA Sequence Homology Soil Microbiology Spartina alterniflora Clone library DGGE Diazotroph Assemblage composition Halophyte Monocotyledones Gramineae Angiospermae Bacteria Spermatophyta Spartina alterniflora
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