Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae ( Danio rerio )

• Published in: Toxics (Basel) Vol. 10; no. 4; p. 198
• Main Authors: Di Paola, Davide, Natale, Sabrina, Iaria, Carmelo, Crupi, Rosalia, Cuzzocrea, Salvatore, Spanò, Nunziacarla, Gugliandolo, Enrico, Peritore, Alessio Filippo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18-04-2022; MDPI
• Subjects: Anthropogenic factors; Antioxidants; Aquatic habitats; Body length; Cadmium; Chemicals; Danio rerio; Defense mechanisms; Developmental stages; Down-regulation; Eggs; Embryos; Endocrine disruptors; environment contaminant; Enzymes; Experiments; Exposure; Fertilization; Fish reproduction; Gene expression; heavy metals; Homeobox; Hormones; Larvae; Lipid peroxidation; Lipids; Morphology; Oxidants; Oxidative stress; Oxidizing agents; Perchlorate; Perchloric acid; Pollutants; Pollution effects; Potassium; Potassium perchlorates; Receptors; Thyroid; thyroid function; Thyroid gland; Thyroxine; Toxicity; Zebrafish; United States > US; thyroid function; environment contaminant; heavy metals; oxidative stress
• ISSN: 2305-6304; 2305-6304
• DOI: 10.3390/toxics10040198

The increasing pollution of aquatic habitats with anthropogenic compounds has led to various test strategies to detect hazardous chemicals. However, information on the effects of pollutants on the thyroid system in fish, which is essential for growth, development, and parts of reproduction, is still scarce. Modified early life-stage tests were carried out with zebrafish exposed to the known thyroid inhibitor potassium perchlorate (0.1, 1, 1.5, 2, 2.5, and 5 mM) to identify adverse effects on embryo development. The endogenous antioxidant defense mechanism is one of the key functions of the thyroid gland; in this regard, we examined the co-exposure to potassium perchlorate (KClO
), which could disrupt thyroid function, with cadmium (Cd), a known pro-oxidant compound. Zebrafish embryos were exposed to control KClO
1 mM and Cd 0.5 μM for 96 h after fertilization (hpf) individually and in combination. The morphological alteration, body length, and messenger RNA (mRNA) expression related to thyroid function and oxidative stress, thyroid hormone levels, and malondialdehyde were measured. Significant down-regulation of mRNAs related to thyroid function (thyroid hormone receptor-alpha (THRα), thyroid hormone receptor-beta (THRβ), haematopoietically expressed homeobox (hhex)) and decreased thyroxin (T4) levels were observed after co-exposure to KClO
and Cd, but this was not observed in the individually treated groups. These results suggest that co-exposure to KClO
and Cd could affect antioxidant defense mechanisms and potentially normally increase Cd toxicity on mRNA expression, altering the thyroid functions important in zebrafish embryonic developmental stages.