Imaging Cells and Tissues with Refractive Index Radiology
Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enh...
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| Published in: | Biophysical journal Vol. 87; no. 6; pp. 4180 - 4187 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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01-12-2004
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| Abstract | Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells. |
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| AbstractList | Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells. [PUBLICATION ABSTRACT] Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells. |
| Author | Ho, P.C. Moser, H.O. Tsai, W.L. Groso, A. Margaritondo, G. Yang, P. Stefanekova, E. Chang, H.M. Yang, Y.C. Chow, G.M. Seol, S.K. Li, S.C. Tsai, H.L. Hwu, Y. Yeh, H.I. Hsu, P.C. Su, Y.T. Kim, C.C. Je, J.H. |
| AuthorAffiliation | Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan; † Pandis Biomedical Research Association, Chupei, Taiwan; ‡ Departments of Medical Research and Internal Medicine, Mackay Memorial Hospital, Mackay Medicine, Nursing and Management College, and Taipei Medical University, Taipei, Taiwan; § Department of Materials Science and Singapore-Massachusetts Institute of Technology Alliance, National University of Singapore, Singapore; ¶ Department of Pharmacy, National University of Singapore, Singapore; ‖ Singapore Synchrotron Light Source, National University of Singapore, Singapore; Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, Korea; and †† Faculté des Sciences de Base, Ecole Polytechnique Fédérale, CH-1015 Lausanne, Switzerland |
| AuthorAffiliation_xml | – name: Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan; † Pandis Biomedical Research Association, Chupei, Taiwan; ‡ Departments of Medical Research and Internal Medicine, Mackay Memorial Hospital, Mackay Medicine, Nursing and Management College, and Taipei Medical University, Taipei, Taiwan; § Department of Materials Science and Singapore-Massachusetts Institute of Technology Alliance, National University of Singapore, Singapore; ¶ Department of Pharmacy, National University of Singapore, Singapore; ‖ Singapore Synchrotron Light Source, National University of Singapore, Singapore; Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, Korea; and †† Faculté des Sciences de Base, Ecole Polytechnique Fédérale, CH-1015 Lausanne, Switzerland |
| Author_xml | – sequence: 1 givenname: Y. surname: Hwu fullname: Hwu, Y. organization: Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan – sequence: 2 givenname: W.L. surname: Tsai fullname: Tsai, W.L. organization: Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan – sequence: 3 givenname: H.M. surname: Chang fullname: Chang, H.M. organization: Pandis Biomedical Research Association, Chupei, Taiwan – sequence: 4 givenname: H.I. surname: Yeh fullname: Yeh, H.I. organization: Departments of Medical Research and Internal Medicine, Mackay Memorial Hospital, Mackay Medicine, Nursing and Management College, and Taipei Medical University, Taipei, Taiwan – sequence: 5 givenname: P.C. surname: Hsu fullname: Hsu, P.C. organization: Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan – sequence: 6 givenname: Y.C. surname: Yang fullname: Yang, Y.C. organization: Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan – sequence: 7 givenname: Y.T. surname: Su fullname: Su, Y.T. organization: Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan – sequence: 8 givenname: H.L. surname: Tsai fullname: Tsai, H.L. organization: Pandis Biomedical Research Association, Chupei, Taiwan – sequence: 9 givenname: G.M. surname: Chow fullname: Chow, G.M. organization: Department of Materials Science and Singapore-Massachusetts Institute of Technology Alliance, National University of Singapore, Singapore – sequence: 10 givenname: P.C. surname: Ho fullname: Ho, P.C. organization: Department of Pharmacy, National University of Singapore, Singapore – sequence: 11 givenname: S.C. surname: Li fullname: Li, S.C. organization: Department of Pharmacy, National University of Singapore, Singapore – sequence: 12 givenname: H.O. surname: Moser fullname: Moser, H.O. organization: Singapore Synchrotron Light Source, National University of Singapore, Singapore – sequence: 13 givenname: P. surname: Yang fullname: Yang, P. organization: Singapore Synchrotron Light Source, National University of Singapore, Singapore – sequence: 14 givenname: S.K. surname: Seol fullname: Seol, S.K. organization: Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, Korea; and – sequence: 15 givenname: C.C. surname: Kim fullname: Kim, C.C. organization: Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, Korea; and – sequence: 16 givenname: J.H. surname: Je fullname: Je, J.H. email: jhje@postech.ac.kr organization: Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, Korea; and – sequence: 17 givenname: E. surname: Stefanekova fullname: Stefanekova, E. organization: Faculté des Sciences de Base, Ecole Polytechnique Fédérale, CH-1015 Lausanne, Switzerland – sequence: 18 givenname: A. surname: Groso fullname: Groso, A. organization: Faculté des Sciences de Base, Ecole Polytechnique Fédérale, CH-1015 Lausanne, Switzerland – sequence: 19 givenname: G. surname: Margaritondo fullname: Margaritondo, G. organization: Faculté des Sciences de Base, Ecole Polytechnique Fédérale, CH-1015 Lausanne, Switzerland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15465870$$D View this record in MEDLINE/PubMed |
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| Copyright | 2004 The Biophysical Society Copyright Biophysical Society Dec 2004 Copyright © 2004, Biophysical Society 2004 |
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| Snippet | Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We... |
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| SubjectTerms | Animals Cells Cells, Cultured - diagnostic imaging Cellular biology Humans Microscopy Radiographic Image Enhancement - methods Radiographic Image Interpretation, Computer-Assisted - methods Radiography - methods Radiology Refractometry - methods Spectroscopy, Imaging, Other Techniques Tissues |
| Title | Imaging Cells and Tissues with Refractive Index Radiology |
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