Real-Time Control of Neutrophil Metabolism by Very Weak Ultra-Low Frequency Pulsed Magnetic Fields

• Published in: Biophysical journal Vol. 88; no. 5; pp. 3334 - 3347
• Main Authors: Rosenspire, Allen J., Kindzelskii, Andrei L., Simon, Bruce J., Petty, Howard R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-05-2005; Biophysical Society
• Subjects: Biophysics; Biophysics - methods; Calcium - metabolism; Cell Membrane - metabolism; Cell Size; Cells; Channels, Receptors, and Electrical Signaling; Electromagnetic Fields; Humans; Magnetic fields; Magnetics; Microscopy, Fluorescence; NADP - chemistry; NADP - metabolism; Neutrophils - cytology; Neutrophils - metabolism; Nitric Oxide - chemistry; Nitric Oxide - metabolism; Oscillometry; Oxidation-Reduction; Reactive Oxygen Species; Signal Transduction; Time Factors
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1529/biophysj.104.056663

In adherent and motile neutrophils NAD(P)H concentration, flavoprotein redox potential, and production of reactive oxygen species and nitric oxide, are all periodic and exhibit defined phase relationships to an underlying metabolic oscillation of ∼20
s. Utilizing fluorescence microscopy, we have shown in real-time, on the single cell level, that the system is sensitive to externally applied periodically pulsed weak magnetic fields matched in frequency to the metabolic oscillation. Depending upon the phase relationship of the magnetic pulses to the metabolic oscillation, the magnetic pulses serve to either increase the amplitude of the NAD(P)H and flavoprotein oscillations, and the rate of production of reactive oxygen species and nitric oxide or, alternatively, collapse the metabolic oscillations and curtail production of reactive oxygen species and nitric oxide. Significantly, we demonstrate that the cells do not directly respond to the magnetic fields, but instead are sensitive to the electric fields which the pulsed magnetic fields induce. These weak electric fields likely tap into an endogenous signaling pathway involving calcium channels in the plasma membrane. We estimate that the threshold which induced electric fields must attain to influence cell metabolism is of the order of 10
−4
V/m.