Selective purification of the 20,000-Da light chains of smooth muscle myosin

The 20,000-Da light chains of gizzard smooth muscle myosin have been purified to homogeneity. Actomyosin, prepared by MgATP extraction of myofibrils, was denatured in 8 M urea, 1 M guanidine HCl, and 0.05% sodium dodecyl sulfate. Myosin heavy chains were precipitated with ethanol and the light chain...

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Bibliographic Details

Published in:	Analytical biochemistry Vol. 135; no. 1; p. 37
Main Authors:	Hathaway, D R, Haeberle, J R
Format:	Journal Article
Language:	English
Published:	United States 01-11-1983
Subjects:	Animals Chickens Chromatography - methods Gizzard, Avian - analysis Muscle, Smooth - analysis Myosins - isolation & purification Turkeys
Online Access:	Get more information
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