Regulation of trophoblast interferon gene expression

• Published in: Molecular and cellular endocrinology Vol. 100; no. 1-2; p. 93
• Main Authors: Flint, A P, Guesdon, F M, Stewart, H J
• Format: Journal Article
• Language: English
• Published: Ireland 01-04-1994
• Subjects: Animals; Blastocyst - physiology; Cattle; CHO Cells; Choriocarcinoma - pathology; Corpus Luteum - physiology; Cricetinae; Cricetulus; Female; Gene Expression Regulation; Interferon Type I - biosynthesis; Interferon Type I - genetics; L Cells; Mice; Organ Specificity; Pregnancy; Pregnancy Proteins - biosynthesis; Pregnancy Proteins - genetics; Pregnancy, Animal - physiology; Promoter Regions, Genetic; Recombinant Fusion Proteins - biosynthesis; Ruminants - physiology; Trophoblasts - metabolism; Tumor Cells, Cultured; Uterine Neoplasms - pathology
• ISSN: 0303-7207
• DOI: 10.1016/0303-7207(94)90285-2

The trophoblast of the developing ruminant conceptus secretes large quantities (up to 100 micrograms, or 10(7) IU, per 24 h) of a number of characteristic Type I interferons. Secretion (gene expression) commences at Day 8 or 9 (in the sheep; Day 10 in cattle) and ends at about Day 22 (Day 25). The function of this material is in the inhibition of uterine prostaglandin secretion, and hence in the maintenance of the corpus luteum (the 'maternal recognition of pregnancy'). Such rapid onset and cessation of gene transcription begs questions about the function of trophoblast interferon gene promoters, and a limited number of studies have now been carried out with reporter gene constructs, with inconclusive results. It seems unlikely, however, that viral responsiveness accounts for the phenomenon, as in adult interferon genes, although the trophoblast interferons are highly active antiviral agents.