Culture of human dental pulp cells at variable times post-tooth extraction

• Published in: Brazilian oral research Vol. 32; p. e003
• Main Authors: Daniela Ferreira Araújo, Benício, Luciana Oliveira, Pereira, Izabel Cristina Rodrigues da, Silva, Ricardo Bentes, Azevedo, Ana Cristina Barreto, Bezerra
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Pesquisa Odontológica - SBPqO 01-02-2018; Sociedade Brasileira de Pesquisa Odontológica
• Subjects: Adult Stem Cells; Dental Pulp; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Mesenchymal Stem Cell; Stem Cell Research; Adult Stem Cells; Mesenchymal Stem Cell; Dental Pulp; Stem Cell Research
• ISSN: 1807-3107; 1807-3107
• DOI: 10.1590/1807-3107bor-2018.vol32.0003

The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.