Isolation and Mapping of Phosphotransferase Mutants in Escherichia coli

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Published in:	Journal of Bacteriology Vol. 104; no. 2; pp. 793 - 797
Main Authors:	Epstein, W, Jewett, S, Fox, C F
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-11-1970
Subjects:	Chromosome Mapping Escherichia coli - enzymology Escherichia coli - isolation & purification Genes Genetics and Molecular Biology Genetics, Microbial Mutation Phosphotransferases - metabolism Recombination, Genetic Transduction, Genetic
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Abstract	Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JB About JB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0021-9193 Online ISSN: 1098-5530 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JB .asm.org, visit: JB
AbstractList	Mutants of Escherichia coli K-12 defective in enzyme I or Hpr, the two common components of the phosphoenolpyruvate-dependent phosphotransferase system, were isolated by a simple, direct method. The ptsI locus, the structural gene for enzyme I, and the ptsH locus, the site of mutations leading to loss of Hpr activity, are adjacent genes and could be part of a single operon. These two genes lie between the purC and supN markers in the order: strA... guaB-purC-ptsI-ptsH-supN-dsdA... his . Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JB About JB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0021-9193 Online ISSN: 1098-5530 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JB .asm.org, visit: JB Mutants of Escherichia coli K-12 defective in enzyme I or Hpr, the two common components of the phosphoenolpyruvate-dependent phosphotransferase system, were isolated by a simple, direct method. The ptsI locus, the structural gene for enzyme I, and the ptsH locus, the site of mutations leading to loss of Hpr activity, are adjacent genes and could be part of a single operon. These two genes lie between the purC and supN markers in the order: strA... guaB-purC-ptsI-ptsH-supN-dsdA... his.
Author	C. Fred Fox Wolfgang Epstein Susan Jewett
AuthorAffiliation	Department of Biochemistry, University of Chicago, Chicago, Illinois 60637
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Cites_doi	10.1128/BR.31.4.332-353.1967 10.1093/genetics/61.2.371 10.1146/annurev.mi.23.100169.002543 10.1128/JB.101.3.836-843.1970 10.1073/pnas.52.4.1067 10.1128/JB.97.1.250-255.1969 10.1128/JB.98.2.605-610.1969 10.1128/JB.102.2.377-381.1970
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References_xml	– volume: 2 start-page: 89 year: 1968 ident: p_2 article-title: Absence of phosphotransferasesystem components in mutant Escherichia coli K-12 with a disrupted carbohydrate-transfer system publication-title: Mol. Biol. contributor: fullname: Burd G. I. – volume: 31 start-page: 332 year: 1967 ident: p_8 article-title: Revised linkage map of Escherichia coll publication-title: Bacteriol. Rev. doi: 10.1128/BR.31.4.332-353.1967 contributor: fullname: Taylor A. L. – volume: 61 start-page: 371 year: 1969 ident: p_7 article-title: Mutagens which cause deletions in Escherichia coll publication-title: Genetics doi: 10.1093/genetics/61.2.371 contributor: fullname: Schwartz D. O. – volume: 23 start-page: 539 year: 1969 ident: p_1 article-title: Carbohydrate metabolism in microorganisms. Annu publication-title: Rev. Microbiol. doi: 10.1146/annurev.mi.23.100169.002543 contributor: fullname: Anderson R. L. – volume: 101 start-page: 836 year: 1970 ident: p_3 article-title: Potassium-dependent mutants of Escherichia colt K-12 publication-title: J. Bacteriol. doi: 10.1128/JB.101.3.836-843.1970 contributor: fullname: Epstein W. – volume: 52 start-page: 1067 year: 1964 ident: p_5 article-title: Phosphate bound to histidine in a protein as an intermediate in a novel phosphotransferase system publication-title: Proc. Nat. Acad. Sci. U.S.A. doi: 10.1073/pnas.52.4.1067 contributor: fullname: Kundig W. – volume: 59 start-page: 9988 year: 1968 ident: p_4 article-title: The role of a phosphoenolpyruvate-dependent kinase system in f-glucoside catabolism in Escherichia coli publication-title: Proc. Nat. Acad. Sci. U.S.A. contributor: fullname: Fox C. F. – volume: 97 start-page: 250 year: 1969 ident: p_6 article-title: Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium publication-title: J. Bacteriol. doi: 10.1128/JB.97.1.250-255.1969 contributor: fullname: Levinthal M. – volume: 98 start-page: 605 year: 1969 ident: p_10 article-title: Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations publication-title: J. Bacteriol. doi: 10.1128/JB.98.2.605-610.1969 contributor: fullname: Wang R. J. – volume: 102 start-page: 377 year: 1970 ident: p_9 article-title: Mapping of the nadB locus adjacent to a previously undescribed purine locus in Escherichia coli K-12 publication-title: J. Bacteriol. doi: 10.1128/JB.102.2.377-381.1970 contributor: fullname: Tritz G. J.
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SubjectTerms	Chromosome Mapping Escherichia coli - enzymology Escherichia coli - isolation & purification Genes Genetics and Molecular Biology Genetics, Microbial Mutation Phosphotransferases - metabolism Recombination, Genetic Transduction, Genetic
Title	Isolation and Mapping of Phosphotransferase Mutants in Escherichia coli
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