Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography

To enhance the quantity and quality of eukaryotic transmembrane proteins (TMPs) available for structure determination by X-ray crystallography, we have optimized protocols for purification of TMPs expressed in the yeast Saccharomyces cerevisiae. We focused on a set of the highest-expressing endogeno...

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Bibliographic Details
Published in:	Protein expression and purification Vol. 71; no. 2; pp. 207 - 223
Main Authors:	Clark, Kathleen M., Fedoriw, Nadia, Robinson, Katrina, Connelly, Sara M., Randles, Joan, Malkowski, Michael G., DeTitta, George T., Dumont, Mark E.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-06-2010
Subjects:	Affinity chromatography Chromatography, Affinity Chromatography, Gel Crystallography Crystallography, X-Ray - methods Detergents Eukaryota - genetics Eukaryota - isolation & purification Eukaryota - metabolism Genetic Vectors Membrane proteins Membrane Proteins - genetics Membrane Proteins - isolation & purification Membrane Proteins - metabolism Protein Binding - genetics Protein expression Rhinovirus Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - isolation & purification Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - isolation & purification Saccharomyces cerevisiae Proteins - metabolism Structural genomics X-Rays Yeast Protein expression Yeast Structural genomics Detergents Crystallography Membrane proteins
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