Combined Toxicity of Xenobiotics Bisphenol A and Heavy Metals on Zebrafish Embryos ( Danio rerio )

• Published in: Toxics (Basel) Vol. 9; no. 12; p. 344
• Main Authors: Di Paola, Davide, Capparucci, Fabiano, Lanteri, Giovanni, Cordaro, Marika, Crupi, Rosalia, Siracusa, Rosalba, D'Amico, Ramona, Fusco, Roberta, Impellizzeri, Daniela, Cuzzocrea, Salvatore, Spanò, Nunziacarla, Gugliandolo, Enrico, Peritore, Alessio Filippo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09-12-2021; MDPI
• Subjects: Antioxidants; Apoptosis; Aquatic organisms; Bisphenol A; Cadmium; Chemical compounds; Chromium; Danio rerio; Developmental stages; Edema; Eggs; Embryos; Enzymes; Exposure; Gene expression; Hatching; Heart rate; Heavy metals; Immune system; Immunotoxicity; Inflammation; Inflammatory response; Larvae; Morphology; Oxidative stress; Pollutants; Survival; Synergistic effect; Toxicity; Xenobiotics; Zebrafish; St Louis Missouri; United States > US; Germany; toxicity; cadmium; chromium; bisphenol A; Danio rerio
• ISSN: 2305-6304; 2305-6304
• DOI: 10.3390/toxics9120344

Environmental pollutants may cause adverse effects on the immune system of aquatic organisms. This study revealed that combination of environmental pollutants and Bisphenol A(BPA) could cause an acute inflammatory response in zebrafish larvae as shown by body alterations, which may imply a common immunotoxicity mechanism for most environmental pollutants. In the present study we evaluated the toxicity after co-exposure of BPA and Cd or Cr (III) in zebrafish embryos and larvae, and the oxidative stress pathway involved. Evaluation of lethal and developmental endpoints such as hatching, edema, malformations, abnormal heart rate and survival rate were evaluated after 96 h of exposure. Combination of BPA at 10 μM with Cd or Cr at 0.5 μM exposure induce malformations at 96 hpf in zebrafish larvae, as well as significantly increases oxidative stress and induce apoptosis on larvae. Our study suggested how environmental pollutant showed a synergistic effect at common not-effective doses, promoting decrease of antioxidant defense and contrasted fish development.