Influence of orally administered CpG-ODNs on the humoral response to bovine serum albumin (BSA) in chickens

• Published in: Veterinary Immunology and Immunopathology Vol. 110; no. 3; pp. 257 - 267
• Main Authors: Ameiss, K.A., Attrache, J. El, Barri, A., McElroy, A.P., Caldwell, D.J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-04-2006
• Subjects: Adjuvant; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - pharmacology; Administration, Oral; Animals; Antibodies; Antibodies - drug effects; Antibodies - immunology; antigens; bovine serum albumin; BSA; Chicken; chickens; Chickens - immunology; CpG-ODN; Dose-Response Relationship, Drug; drinking water; ELISA; food animals; humoral immunity; immune response; immunization; immunoglobulin A; Immunoglobulin A - blood; immunoglobulin G; Immunoglobulin G - blood; immunoglobulin M; Immunoglobulin M - blood; Immunomodulation; intestinal mucosa; Intestines - drug effects; Intestines - immunology; Male; mucosal immunity; nucleotides; oligodeoxynucleotides; Oligodeoxyribonucleotides - administration & dosage; Oligodeoxyribonucleotides - pharmacology; Oral immunization; Serum Albumin, Bovine - immunology; Time Factors; vaccine adjuvants; BSA; Immunomodulation; CpG-ODN; Antibodies; Chicken; Adjuvant; Oral immunization; ELISA
• ISSN: 0165-2427; 1873-2534; 1365-2567
• DOI: 10.1016/j.vetimm.2005.10.011

Synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODNs) have been reported to be effective mucosal adjuvants in mice when given orally. Studies on their effectiveness in chickens are currently very limited. This study investigated whether CpG-ODNs could adjuvant the immune response to BSA when given orally to a commercial line of SCWL chickens. In two experiments, performed over time, chickens were given selected concentrations of CpG-ODNs with BSA followed by 6 consecutive days of ad libitum access to drinking water containing 1.4
mg/ml BSA. Serum responses, and in some cases intestinal specific antibodies, were measured out to 33 days post-immunization. Birds receiving a single dose of CpG-ODN had consistently higher IgG, IgM, and IgA titers in the serum, dependent upon dose, and in specific areas of the intestine when compared to the non-immunized and BSA only groups. These findings suggest that a single oral CpG-ODN administration can accelerate the kinetics of antigen specific antibodies of all three isotypes in commercial-strain chickens immunized via the drinking water using common protein antigen.