The Membrane Proteome of Spores and Vegetative Cells of the Food-Borne Pathogen Bacillus cereus

• Published in: International journal of molecular sciences Vol. 22; no. 22; p. 12475
• Main Authors: Gao, Xiaowei, Swarge, Bhagyashree N, Dekker, Henk L, Roseboom, Winfried, Brul, Stanley, Kramer, Gertjan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19-11-2021; MDPI
• Subjects: Algorithms; Bacillus cereus; Bacillus cereus - genetics; Bacillus cereus - pathogenicity; Bacterial Proteins - classification; Bacterial Proteins - genetics; Bioinformatics; Carbohydrates; Cell division; Cell Membrane - genetics; Cell membranes; Food; Food Contamination; Food Microbiology; Foodborne Diseases - genetics; Foodborne Diseases - microbiology; Germination; Humans; Localization; Mass spectrometry; Membrane proteins; Membrane Proteins - classification; Membrane Proteins - genetics; Metabolism; Metabolites; Motility; Pathogens; Protein transport; Proteins; proteome; Proteome - genetics; Proteomes; Proteomics; Scientific imaging; Signal transduction; Spore germination; spore inner membrane; Spores; Spores, Bacterial - genetics; Spores, Bacterial - growth & development; Spores, Bacterial - pathogenicity; Therapeutic targets; Vegetative cells; spore inner membrane; Bacillus cereus; proteome
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222212475

Membrane proteins are fascinating since they play an important role in diverse cellular functions and constitute many drug targets. Membrane proteins are challenging to analyze. The spore, the most resistant form of known life, harbors a compressed inner membrane. This membrane acts not only as a barrier for undesired molecules but also as a scaffold for proteins involved in signal transduction and the transport of metabolites during spore germination and subsequent vegetative growth. In this study, we adapted a membrane enrichment method to study the membrane proteome of spores and cells of the food-borne pathogen
using quantitative proteomics. Using bioinformatics filtering we identify and quantify 498 vegetative cell membrane proteins and 244 spore inner membrane proteins. Comparison of vegetative and spore membrane proteins showed there were 54 spore membrane-specific and 308 cell membrane-specific proteins. Functional characterization of these proteins showed that the cell membrane proteome has a far larger number of transporters, receptors and proteins related to cell division and motility. This was also reflected in the much higher expression level of many of these proteins in the cellular membrane for those proteins that were in common with the spore inner membrane. The spore inner membrane had specific expression of several germinant receptors and spore-specific proteins, but also seemed to show a preference towards the use of simple carbohydrates like glucose and fructose owing to only expressing transporters for these. These results show the differences in membrane proteome composition and show us the specific proteins necessary in the inner membrane of a dormant spore of this toxigenic spore-forming bacterium to survive adverse conditions.