A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes

• Published in: European journal of clinical nutrition Vol. 59; no. 5; pp. 727 - 730
• Main Authors: LEGER, C. L, CARBONNEAU, M. A, MICHEL, F, MAS, E, MONNIER, L, CRISTOL, J. P, DESCOMPS, B
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 01-05-2005; Nature Publishing Group
• Subjects: Adult; Aged; Albumins; Antioxidants; Antioxidants - pharmacology; Ascorbic acid; Bilirubin; Biological and medical sciences; Blood; Blood coagulation; Blood glucose; Carotene; Cholesterol; Clinical nutrition; Clotting; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - drug therapy; Diabetes Mellitus, Type 1 - urine; Diabetes. Impaired glucose tolerance; Dinoprost - analogs & derivatives; Dinoprost - urine; Dose-Response Relationship, Drug; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Food industries wastewaters; High density lipoprotein; Humans; Male; Management. Various non-drug treatments. Langerhans islet grafts; Medical sciences; Middle Aged; Oils & fats; Olive Oil; Phenolic compounds; Phenols; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacology; Plant Oils - pharmacology; Plasma; Platelet Aggregation - drug effects; Prevention; Thromboxane B2 - blood; Time Factors; Triglycerides; Uric acid; Vitamin A; Waste Disposal, Fluid - methods; Waste Products; Wastewater; β-Carotene; Endocrinopathy; Human; Immunopathology; Nutrition; Arachidonic acid derivatives; Autoimmune disease; Patient; Extract; Olive oil; Antioxidant; Olive; TXB2; Type 1 diabetes; Eicosanoid; Thromboxane; hydroxytyrosol; diabetes
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/sj.ejcn.1602133

To assess the antioxidant/non-antioxidant effects of a hydroxytyrosol (HT)-rich phenolic extract from olive mill wastewaters administered with a breakfast.
Five type I diabetic patients received 25 mg of HT the first day and 12.5 mg/day the following 3 days. Blood sampling was carried out at T(0) (baseline) and T(4d) just before the breakfast + HT administration and at time points 1, 2, 3 and 4 h after T(0). Urines (24-h) were collected from T(0) to T(4d). Baseline HbA1c was generally inferior to 10%, glycemia was within the range 6-24 mmol/l, whereas total cholesterol, HDL-chol and triglycerides were normal.
The major finding was the 46% decrease in the serum TXB(2) production after blood clotting at T(4d). Plasma vitamin A, E, beta-carotene were not changed. Vitamin C tended to increase (P = 0.075). Plasma antioxidant capacity was enhanced at T(0)+1 h only, whereas its main determinants (albumin, bilirubin, uric acid) were not modified. Urinary 8-isoPGF(2alpha) levels were highly variable and were not affected significantly by HT administration.
The major effect of HT accounts for an antiaggregating platelet action, leading to a possible prevention of thrombotic and microthrombotic processes.