A serotonin voltammetric biosensor composed of carbon nanocomposites and DNA aptamer

• Published in: Mikrochimica acta (1966) Vol. 188; no. 4; p. 146
• Main Authors: Li, Jingjing, Si, Yunpei, Park, Yae Eun, Choi, Jung-Seok, Jung, Sung Mi, Lee, Ji Eun, Lee, Hye Jin
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-04-2021; Springer; Springer Nature B.V
• Subjects: Agaricales - enzymology; Analysis; Analytical Chemistry; Aptamers, Nucleotide - chemistry; Biosensing Techniques - methods; Biosensors; Buffer solutions; Carbon; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Detectors; DNA; DNA - chemistry; Electric properties; Electrochemical impedance spectroscopy; Electrochemical Techniques - methods; Enzymes, Immobilized - chemistry; Gold; Gold - chemistry; Humans; Internet Addiction Disorder - blood; Internet Addiction Disorder - diagnosis; Limit of Detection; Liquid chromatography; Mass spectrometry; Metal Nanoparticles - chemistry; Microengineering; Monophenol Monooxygenase - chemistry; Nanochemistry; Nanocomposites; Nanocomposites - chemistry; Nanoparticles; Nanotechnology; Nanotubes; Nanotubes, Carbon - chemistry; Phenols; Photoelectrons; Polyethylenes - chemistry; Quaternary Ammonium Compounds - chemistry; Self-assembly; Serotonin; Serotonin - blood; Serotonin - chemistry; Short Communication; Silver chloride; Single wall carbon nanotubes; Spectrometry; Spectrum analysis; Surface properties; Tyrosinase; Voltammetry; X-ray spectroscopy; Voltammetric biosensor; Serotonin-specific aptamer; Serotonin; Internet gaming disorder; Single-walled carbon nanotubes; Tyrosinase
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-021-04798-x

A sensitive and selective voltammetric biosensor composed of layer-by-layer (LbL) self-assembly of positively charged poly(diallyldimethylammonium)-wrapped oxidized single-walled carbon nanotubes (PDDA-oSWCNTs), negatively charged serotonin (5-hydroxytryptamine, 5-HT)-specific aptamer, and tyrosinase on Au nanoparticles deposited screen printed carbon electrode was developed for measurement of 5-HT. Surface characteristics of 5-HT biosensor were explored using scanning electron microscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. The respective effects of 5-HT-specific aptamer and oSWCNTs on the detection of 5-HT were investigated by differential pulse voltammetry (DPV). The peak current at the potential of 0.29 V (vs. Ag/AgCl) increased with respect to 5-HT concentration resulting in two dynamic ranges from 0.05 to 0.5 and 1 to 20 μM with a limit of detection of 2 nM from the LbL biosensor in buffer solution, which were better than those without the LbL of aptamer and oSWCNTs. The developed biosensor was applied to the direct determination of 5-HT concentrations in undiluted healthy control and Internet gaming disorder serum samples. The results were verified by comparison with those from liquid chromatography-mass spectrometric analyses.
Graphical abstract