Overexpression of the Trichoderma brevicompactum tri5 gene: effect on the expression of the trichodermin biosynthetic genes and on tomato seedlings

• Published in: Toxins Vol. 3; no. 9; pp. 1220 - 1232
• Main Authors: Tijerino, Anamariela, Hermosa, Rosa, Cardoza, Rosa E, Moraga, Javier, Malmierca, Monica G, Aleu, Josefina, Collado, Isidro G, Monte, Enrique, Gutierrez, Santiago
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-09-2011; MDPI
• Subjects: Antioxidants - pharmacology; Biosynthesis; ergosterol; gene expression; Gene Expression Regulation, Fungal - drug effects; Genes, Fungal - genetics; Lycopersicon esculentum - drug effects; Lycopersicon esculentum - growth & development; Lycopersicon esculentum - microbiology; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacology; Seedlings; Seedlings - drug effects; Seedlings - growth & development; Seedlings - microbiology; terpene; Tomatoes; Toxins; tri genes; Trichoderma - genetics; Trichodermin - metabolism; Trichodermin - toxicity; trichothecene; tyrosol; tyrosol; terpene; tri genes; trichothecene; gene expression; ergosterol
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins3091220

Trichoderma brevicompactum IBT 40841 produces trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.