Gene expression, function, and diversity of Nkx2-4 in the rainbow trout, Oncorhynchus mykiss

• Published in: General and comparative endocrinology Vol. 206; pp. 193 - 202
• Main Authors: Uemae, Youji, Sakamoto, Joe, Hidaka, Yoshie, Hiratsuka, Ai, Susa, Takao, Kato, Yukio, Suzuki, Masakazu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-09-2014
• Subjects: Amino Acid Sequence; Animals; Cloning, Molecular; DNA, Complementary - genetics; Electrophoretic Mobility Shift Assay; Endocrinology & Metabolism; Gene Expression Regulation; In Situ Hybridization; Molecular Sequence Data; Nkx2-1; Nkx2-4; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oncorhynchus mykiss - metabolism; Ovary; Phylogeny; Protein Isoforms - metabolism; Rainbow trout; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Thyroglobulin - metabolism; Thyroid Nuclear Factor 1; Thyroid transcription factor; Tissue Distribution; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptional Activation; TTF-1; Ovary; Nkx2-4; Thyroid transcription factor; TTF-1; Nkx2-1; Rainbow trout
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2014.07.007

Highlights • We provide the first evidence for Nkx2-4 mRNAs in vertebrate thyroid follicles. • Three types of Nkx2-4 mRNAs (a–c) were found in the rainbow trout thyroid. • Nkx2-4a and Nkx2-4b directly transactivated thyroglobulin gene expression. • Nkx2-4c did not show a significant increase in the thyroglobulin expression. • The results are important for understanding gene regulation in the fish thyroid.