Differential T-Cell Reactivity to Endemic Coronaviruses and SARS-CoV-2 in Community and Health Care Workers

• Published in: The Journal of infectious diseases Vol. 224; no. 1; pp. 70 - 80
• Main Authors: da Silva Antunes, Ricardo, Pallikkuth, Suresh, Williams, Erin, Dawen Yu, Esther, Mateus, Jose, Quiambao, Lorenzo, Wang, Eric, Rawlings, Stephen A, Stadlbauer, Daniel, Jiang, Kaijun, Amanat, Fatima, Arnold, David, Andrews, David, Fuego, Irma, Dan, Jennifer M, Grifoni, Alba, Weiskopf, Daniela, Krammer, Florian, Crotty, Shane, Hoffer, Michael E, Pahwa, Savita G, Sette, Alessandro
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 02-07-2021
• Subjects: Adult; Antibodies, Viral; Biomarkers; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; Cell activation; Common cold; Coronaviridae; Coronaviruses; COVID-19; COVID-19 - diagnosis; COVID-19 - epidemiology; COVID-19 - immunology; COVID-19 - virology; Cross-reactivity; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte - chemistry; Epitopes, T-Lymphocyte - immunology; Female; Health care; Health Personnel; Humans; Immunophenotyping; Lymphocyte Activation - immunology; Lymphocytes T; Major and Brief Reports; Male; Medical personnel; Middle Aged; Occupational exposure; Peptides - chemistry; Peptides - immunology; Public Health Surveillance; SARS-CoV-2 - immunology; Seroepidemiologic Studies; Severe acute respiratory syndrome coronavirus 2; Severity of Illness Index; Spike Glycoprotein, Coronavirus - immunology; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism; COVID-19; SARS-CoV-2; coronaviruses; health care workers; T cells
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/jiab176

Abstract
Herein we measured CD4+ T-cell responses against common cold coronaviruses (CCC) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in high-risk health care workers (HCW) and community controls. We observed higher levels of CCC-reactive T cells in SARS-CoV-2–seronegative HCW compared to community donors, consistent with potential higher occupational exposure of HCW to CCC. We further show that SARS-CoV-2 T-cell reactivity of seronegative HCW was higher than community controls and correlation between CCC and SARS-CoV-2 responses is consistent with cross-reactivity and not associated with recent in vivo activation. Surprisingly, CCC T-cell reactivity was decreased in SARS-CoV-2–infected HCW, suggesting that exposure to SARS-CoV-2 might interfere with CCC responses, either directly or indirectly. This result was unexpected, but consistently detected in independent cohorts derived from Miami and San Diego.
CD4+ T-cell responses against common cold coronaviruses (CCC) are elevated in SARS-CoV-2 seronegative high-risk health care workers (HCW) compared to COVID-19 convalescent HCW, suggesting that exposure to SARS-CoV-2 might interfere with CCC responses and/or cross-reactivity associated with a protective effect.
CD4+ T-cell responses against common cold coronaviruses (CCC) are elevated in SARS-CoV-2 seronegative high-risk health care workers (HCW) compared to COVID-19 convalescent HCW, suggesting that exposure to SARS-CoV-2 might interfere with CCC responses and/or cross-reactivity a protective effect.