Genetic diversity and effect of temperature and pH on the growth of Macrophomina phaseolina isolates from sunflower fields in Hungary

• Published in: Molecular biology reports Vol. 39; no. 3; pp. 3259 - 3269
• Main Authors: Csöndes, Izabella, Cseh, András, Taller, János, Poczai, Péter
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-03-2012; Springer Nature B.V
• Subjects: Agar; Animal Anatomy; Animal Biochemistry; Ascomycota - genetics; Ascomycota - growth & development; Biomedical and Life Sciences; Cluster Analysis; Coexistence; Data processing; dextrose; DNA Primers - genetics; DNA, Ribosomal - genetics; Dominance; Endonuclease; Fungi; Genetic distance; Genetic diversity; Genetic relationship; Genetic Variation; Geography; Haplotypes; Haplotypes - genetics; Helianthus; Helianthus - microbiology; Histology; Hungary; Hydrogen-Ion Concentration; Life Sciences; Macrophomina phaseolina; Morphology; Pathogens; pH effects; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Primers; Random amplified polymorphic DNA; Random Amplified Polymorphic DNA Technique; Solanum tuberosum; Spacer; Temperature; Temperature effects; Hungary; Internal transcribed spacer (ITS); Population structure; Genetic differentiation; Nuclear ribosomal (rDNA) regions; Charcoal rot disease
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-011-1094-6

The effects of temperature and pH on the growth of 45 Hungarian
Macrophomina phaseolina
isolates from different locations and hosts were compared on the basis of their genetic diversity. One Spanish and two Serbian isolates were also included in the experiment. The most favourable temperature regimes for the development of the isolates ranged between 25 and 35°C. The optimal pH for the pathogen varied between 4.0 and 6.0, but growth was observed on potato dextrose agar even at pH values of 3.0, 7.0 and 8.0. RAPD analysis with 13 different primer pairs generated 148 unambiguous bands. RFLP analysis involving 8 different restriction endonucleases was performed on a 1550 bp fragment of the rDNA region containing internal transcribed spacers (ITS1, ITS2), the 5.8S rDNA and part of the 25S rDNA. The greatest genetic distance values were obtained for three isolates, two from Hungary and one from Spain, which had similar values, but were quite distinct from all the others. A strong positive correlation was observed between the genetic distances and the growth parameters measured at various temperatures, and between the geographical data and the growth data sets at different pH values, but the correlation was less strong in the latter case. While Hungarian
M. phaseolina
populations are thought to reproduce clonally, the present results indicate the coexistence of different haplotypes in this area, and besides the geographical dominance of a given haplotype it was found that a closer genetic relationship might exist between spatially distinct haplotypes.