Effect of cadmium on photosynthetic pigments, lipid peroxidation, antioxidants, and artemisinin in hydroponically grown Artemisia annua

• Published in: Journal of environmental sciences (China) Vol. 24; no. 8; pp. 1511 - 1518
• Main Authors: Li, Xuan, Zhao, Manxi, Guo, Lanping, Huang, Luqi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-08-2012; School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China; Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China%Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China
• Subjects: antioxidants; Artemisia annua; Artemisia annua - drug effects; Artemisia annua - metabolism; artemisinin; Artemisinins - metabolism; ascorbic acid; Ascorbic Acid - metabolism; cadmium; Cadmium - toxicity; carotenoids; cell membranes; chlorophyll; Electric Conductivity; electrolytes; glutathione; Glutathione - metabolism; Hydroponics; lipid peroxidation; Lipid Peroxidation - drug effects; malondialdehyde; Malondialdehyde - metabolism; oxidative stress; Photosynthesis - drug effects; photosynthetic pigments; pigments; 光合色素; 抗氧化剂; 水栽培; 治疗时间; 脂质过氧化; 还原型谷胱甘肽; 镉（Cd）; 青蒿素; artemisinin; cadmium; Artemisia annua; oxidative stress; photosynthetic pigments
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/S1001-0742(11)60920-0

The effects of different cadmium (Cd) concentrations (0, 20, 60, and 100 μmol/L) on hydroponically grown Artemisia annua L. were investigated. Cd treatments applied for 0, 4, 12, 24, 72, 144, 216, and 336 hr were assessed by measuring the changes in photosynthetic pigments, electrolyte leakage, malondialdehyde (MDA) and antioxidants (ascorbic acid and glutathione), while the artemisinin content was tested after 0, 12, 144, 216, and 336 hr. A significant decrease was observed in photosynthetic pigment levels over time with increasing Cd concentration. Chlorophyll b levels were more affected by Cd than were chlorophyll α or carotenoid levels. The cell membrane was sensitive to Cd stress, as MDA content in all treatment groups showed insignificant differences from the control group, except at 12 hr treatment time. Ascorbic acid (AsA) content changed slightly over time, while glutathione (GSH) content took less time to reach a maximum as Cd concentration increased. Cd was found to promote synthesis and accumulation of artemisinin, especially at concentrations of 20 and 100 μmol/L. In conclusion, Cd stress can damage to photosynthetic pigments, and vigorously growing A. annua showed a strong tolerance for Cd stress. Appropriate amounts of added Cd aided synthesis and accumulation of artemisinin.