Platelet Extracellular Vesicles Drive Inflammasome-IL-1β-Dependent Lung Injury in Sickle Cell Disease

• Published in: American journal of respiratory and critical care medicine Vol. 201; no. 1; pp. 33 - 46
• Main Authors: Vats, Ravi, Brzoska, Tomasz, Bennewitz, Margaret F, Jimenez, Maritza A, Pradhan-Sundd, Tirthadipa, Tutuncuoglu, Egemen, Jonassaint, Jude, Gutierrez, Edgar, Watkins, Simon C, Shiva, Sruti, Scott, Melanie J, Morelli, Adrian E, Neal, Matthew D, Kato, Gregory J, Gladwin, Mark T, Sundd, Prithu
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 01-01-2020
• Subjects: Acute Chest Syndrome - etiology; Acute Chest Syndrome - physiopathology; Anemia, Sickle Cell - complications; Anemia, Sickle Cell - immunology; Anemia, Sickle Cell - physiopathology; Animals; Extracellular vesicles; Extracellular Vesicles - immunology; Humans; Immune system; Inflammasomes - immunology; Lung diseases; Lung Injury - etiology; Lung Injury - physiopathology; Mice; Mice, Transgenic; Models, Animal; Neutrophils - immunology; Original; Platelet Aggregation - immunology; Sickle cell disease; neutrophil–platelet aggregates; acute chest syndrome; vasoocclusion
• ISSN: 1073-449X; 1535-4970
• DOI: 10.1164/rccm.201807-1370OC

Intraerythrocytic polymerization of Hb S promotes hemolysis and vasoocclusive events in the microvasculature of patients with sickle cell disease (SCD). Although platelet-neutrophil aggregate-dependent vasoocclusion is known to occur in the lung and contribute to acute chest syndrome, the etiological mechanisms that trigger acute chest syndrome are largely unknown.
To identify the innate immune mechanism that promotes platelet-neutrophil aggregate-dependent lung vasoocclusion and injury in SCD.
imaging of the lung in transgenic humanized SCD mice and
imaging of SCD patient blood flowing through a microfluidic system was performed. SCD mice were systemically challenged with nanogram quantities of LPS to trigger lung vasoocclusion.
Platelet-inflammasome activation led to generation of IL-1β and caspase-1-carrying platelet extracellular vesicles (EVs) that bind to neutrophils and promote platelet-neutrophil aggregation in lung arterioles of SCD mice
and SCD human blood in microfluidics
. The inflammasome activation, platelet EV generation, and platelet-neutrophil aggregation were enhanced by the presence of LPS at a nanogram dose in SCD but not control human blood. Inhibition of the inflammasome effector caspase-1 or IL-1β pathway attenuated platelet EV generation, prevented platelet-neutrophil aggregation, and restored microvascular blood flow in lung arterioles of SCD mice
and SCD human blood in microfluidics
.
These results are the first to identify that platelet-inflammasome-dependent shedding of IL-1β and caspase-1-carrying platelet EVs promote lung vasoocclusion in SCD. The current findings also highlight the therapeutic potential of targeting the platelet-inflammasome-dependent innate immune pathway to prevent acute chest syndrome.