SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella

• Published in: Microbial pathogenesis Vol. 38; no. 4; pp. 181 - 187
• Main Authors: Carlson, Steve A., McCuddin, Zoe P., Wu, Max T.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier India Pvt Ltd 01-04-2005; Elsevier
• Subjects: Amino Acid Sequence; animal pathogenic bacteria; Animals; Antibiotic resistance; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Base Sequence; Biological and medical sciences; calves; Cattle; Cattle Diseases - microbiology; Cell Line; collagenase; Collagenases - genetics; Collagenases - metabolism; Cytopathic; cytopathic phenotypes; DNA Transposable Elements - genetics; Drug Resistance, Multiple, Bacterial; DT104; food pathogens; Fundamental and applied biological sciences. Psychology; Humans; Integrons; Microbiology; Miscellaneous; Molecular Sequence Data; multiple drug resistance; Mutagenesis, Insertional; phenotype; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; Salmonella; Salmonella enterica; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella Infections, Animal - microbiology; Salmonella typhimurium - enzymology; Salmonella typhimurium - genetics; Salmonella typhimurium - growth & development; Sequence Alignment; Transcription Factors - genetics; Transcription Factors - metabolism; veal; DT104; Salmonella; Cytopathic; Antibiotic resistance; Microbiology; Enzyme; Metalloendopeptidases; Peptidases; Antibiotic; Regulation(control); Multiple resistance; Bacteria; Hydrolases; Interstitial collagenase; Enterobacteriaceae
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2005.01.004

Salmonella enterica serotype Typhimurium phagetype DT104 (DT104) is a foodborne pathogen with a multiresistant phenotype conferred by a genomic-based integron structure designated as SGI1. Recently, a novel cytopathic phenotype was ascribed to several isolates of DT104 recovered from veal calves. This phenotype is dependent upon
clg, a gene encoding a collagenase in
Salmonella. Using a novel transposon system and an RT-PCR assay for detection of
clg expression, we identified SlyA as a regulator of the collagenase-mediated phenotype. The function of SlyA, in regards to
clg expression, is to repress the synthesis of Clg. Derepression ensued in the absence of SlyA or in the presence of a truncated version of SlyA with the latter being relevant for maintenance of another virulence aspect mediated by SlyA, i.e. survival within macrophages. The SlyA-mediated effect on
clg expression was restricted to DT104 and other
Salmonella phagetypes and serotypes possessing SGI1 thus suggesting co-regulation by an SGI1-specific component.