Transcriptomic responses of European flounder ( Platichthys flesus) to model toxicants

• Published in: Aquatic toxicology Vol. 90; no. 2; pp. 83 - 91
• Main Authors: Williams, Tim D., Diab, Amer, Ortega, Fernando, Sabine, Victoria S., Godfrey, Rita E., Falciani, Francesco, Chipman, J. Kevin, George, Stephen G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11-11-2008; Amsterdam; New York: Elsevier Science; Elsevier
• Subjects: acute toxicity; Agnatha. Pisces; Animal, plant and microbial ecology; Animals; apoptosis; Applied ecology; Biological and medical sciences; cadmium; chlorinated hydrocarbons; Ecotoxicogenomics; Ecotoxicology, biological effects of pollution; Fish; Flounder; Flounder - physiology; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation - drug effects; General aspects; Genes - genetics; genomics; genotoxicity; Lindane; liver; Liver - drug effects; Marine; mechanism of action; Microarray; microarray technology; Oligonucleotide Array Sequence Analysis; Oxidative stress; PCB; Peroxisome proliferators; peroxisomes; physiological response; Platichthys flesus; Polycyclic aromatic hydrocarbon; polycyclic aromatic hydrocarbons; Principal Component Analysis; protein synthesis; Time Factors; transcriptomics; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Water Pollutants, Chemical - toxicity; Europe; AN, Atlantic; Cd; Oxidative stress; MIAME; Peroxisome proliferators; Polycyclic aromatic hydrocarbon; Lindane; Ecotoxicogenomics; Microarray; Flounder; PCB; GENIPOL; Fish; OSPAR; SAM; Insecticide; Toxicity; Platichthys flesus; Freshwater environment; Marine environment; Persistent organic pollutant; Aquatic environment; Organochlorine compounds; Chlorine Organic compounds; Pisces; Organic compounds; Cadmium; Ecotoxicology; Hydrocarbon; Pesticides; Polychlorobiphenyls; Polycyclic aromatic compound; Heavy metal; Vertebrata; Peroxisome; Poison; Models
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2008.07.019

The temporal transcriptomic responses in liver of
Platichthys flesus to model environmental pollutants were studied over a 16-day time span after intraperitoneal injection with cadmium chloride (50
μg/kg in saline), 3-methylcholanthrene (25
mg/kg in olive oil), Aroclor 1254 (50
mg/kg in olive oil),
tert-butyl-hydroperoxide (5
mg/kg in saline), Lindane (25
mg/kg in olive oil), perfluoro-octanoic acid (100
mg/kg in olive oil) and their vehicles, olive oil (1
ml/kg) or saline (0.9%). Statistical, gene ontology and supervised analysis clearly demonstrated the progression from acute effects, biological responses to and recovery from the treatments. Key biological processes disturbed by the individual treatments were characterised by gene ontology analyses and individual toxicant-responsive genes and pathways were identified by supervised analyses. Responses to the polyaromatic and chlorinated aromatic compounds showed a degree of commonality but were distinguishable and they were clearly segregated from the responses to the pro-oxidants cadmium and the organic hydroperoxide, as well as from the peroxisomal proliferator, perfluoro-octanoic acid. This study demonstrated the utility of the microarray technique in the identification of toxicant-responsive genes and in discrimination between modes of toxicant action.