Interleukin‐8 Gene Promoter Polymorphism (rs4073) May Contribute to Chronic Periodontitis

• Published in: Journal of periodontology (1970) Vol. 82; no. 6; pp. 893 - 899
• Main Authors: Andia, Denise C., de Oliveira, Naila F. P., Letra, Ariadne M., Nociti, Francisco H., Line, Sergio R. P., de Souza, Ana P.
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Academy of Periodontology 01-06-2011
• Subjects: Adult; Alleles; Biological and medical sciences; Brazil; Case-Control Studies; Chronic Periodontitis - genetics; Dentistry; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; Gene Frequency; genotype; Haplotypes; Humans; Interleukin-8 - biosynthesis; Interleukin-8 - genetics; interleukin‐8; Lipopolysaccharides - immunology; Logistic Models; Male; Medical sciences; Middle Aged; Non tumoral diseases; Odds Ratio; Otorhinolaryngology. Stomatology; periodontitis; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics; Reverse Transcriptase Polymerase Chain Reaction; Statistics, Nonparametric; Up-Regulation; Brazil; Typing; Stomatology; Genotype; Dentistry; interleukin-8; Periodontal disease; Chronic; Microbiological investigation; Gene; Periodontitis; Alleles; single nucleotide; Interleukin 8; Polymorphism
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2010.100513

Background: The proinflammatory chemokine interleukin (IL)‐8 is important in the regulation of the inflammatory response. Analyses of the single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that the A allele upregulated IL‐8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis.
Methods: Genotyping was performed by a standard polymerase chain reaction–restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control subjects and patients with chronic periodontitis; analyses were adjusted by multivariate logistic regression modeling. A real‐time polymerase chain reaction performance was used to detect levels of the IL‐8 mRNA.
Results: The analysis pointed to a statistically significant association of chronic periodontitis with the heterozygous TA genotype (P = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control group versus 48% in the periodontitis group). The higher levels of the IL‐8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype (P = 0.03).
Conclusion: The SNP rs4073 was associated with chronic periodontitis in non‐smoker Brazilian subjects because the frequency of the A allele was higher in the disease group than in the control group, and the TA genotype was associated with increased levels of IL‐8 mRNA transcripts.