Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in situ hybridization and DNA flow cytometry using paraffin‐embedded tissue

• Published in: Cancer Vol. 79; no. 2; pp. 284 - 293
• Main Authors: Aoki, Takatoshi, Hisaoka, Masanori, Kouho, Hiromi, Hashimoto, Hiroshi, Nakata, Hajime
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 15-01-1997; Wiley-Liss
• Subjects: Adult; Aged; Biological and medical sciences; Chromosome Aberrations - genetics; Chromosomes, Human, Pair 12 - genetics; Chromosomes, Human, Pair 16 - genetics; Dermatology; DNA flow cytometry; DNA, Neoplasm - analysis; Female; Flow Cytometry; fluorescence in situ hybridization; Histiocytoma, Benign Fibrous - genetics; Histiocytoma, Benign Fibrous - pathology; Humans; In Situ Hybridization, Fluorescence; Interphase - genetics; intramuscular myxoma; Investigative techniques, diagnostic techniques (general aspects); Liposarcoma, Myxoid - genetics; Liposarcoma, Myxoid - pathology; low grade fibromyxoid sarcoma; Male; Medical sciences; Middle Aged; Muscle Neoplasms - genetics; myxoid liposarcoma; myxoid malignant fibrous histiocytoma; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Soft Tissue; Soft Tissue Neoplasms - genetics; Soft Tissue Neoplasms - pathology; Chromosomal aberration; Human; Flow cytometry; Molecular hybridization; Sarcoma; Interphase; In situ; Fluorescence; Histological preparation; Malignant tumor; Paraffin; Differential diagnostic; Myxoma; Striated muscle disease; Malignant histiocytoma; Liposarcoma; Abnormal chromosome; Genetics; Tumor; Soft tissue; Adipose tissue disorders; Abnormal chromosome C12; Molecular biology; Chromosome translocation
• ISSN: 0008-543X; 1097-0142
• DOI: 10.1002/(SICI)1097-0142(19970115)79:2<284::AID-CNCR12>3.0.CO;2-X

BACKGROUND
Myxoid liposarcoma is one of the major myxoid malignancies of soft tissue and its histologic differentiation from other myxoid tumors is sometimes difficult. Recent cytogenetic analysis revealed that a characteristic reciprocal chromosomal translocation of t(12;16)(q13;p11) occurs in myxoid liposarcomas. In this study, retrospective cytogenetic analysis by means of fluorescence in situ hybridization (FISH) and DNA flow cytometry were performed on free nuclei isolated from paraffin embedded myxoid liposarcoma cells, and they were compared with myxoid malignant fibrous histiocytomas (MFHs), low grade fibromyxoid sarcomas, and intramuscular myxomas.
METHODS
Nine myxoid liposarcomas, five myxoid MFHs, two low grade fibromyxoid sarcomas, and four intramuscular myxomas were examined. Chromosomal aberrations in chromosomes 12 and 16 were investigated by FISH, using both centromeric DNA probes and whole chromosome painting probes. Cellular DNA contents were determined by flow cytometry.
RESULTS
All nine myxoid liposarcomas were shown to be diploid by DNA flow cytometry and exhibited the translocation t(12;16), as shown by FISH. Four of the five myxoid MFHs exhibited aneuploidy, as shown by flow cytometry, and remarkable numeric aberrations of chromosomes 12 and 16. No abnormal DNA content or chromosomal aberrations were identified in the two low grade fibromyxoid sarcomas or the four intramuscular myxomas.
CONCLUSIONS
FISH analysis performed on paraffin embedded materials can be used to differentiate myxoid liposarcomas from other myxoid soft tissue tumors. Cancer 1997; 79:284‐93. © 1997 American Cancer Society.
Fluorescence in situ hybridization analysis can be used on paraffin embedded materials, and is a useful method for differentiating myxoid liposarcomas from other myxoid soft tissue tumors.