Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids

Advances are needed in the site-directed mutagenesis of large plasmids for protein structure-function studies, as current methods are often inefficient, complicated and time-consuming. Here two new methods are reported that overcome these difficulties, namely the single primer extension reaction (SS...

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Bibliographic Details
Published in:	New biotechnology Vol. 72; no. C; pp. 22 - 28
Main Authors:	Jia, Huiyong, Couto-Rodriguez, Ricardo, Johnson, Sharon, Medina, Stephanie, Novillo, Brianna, Huynh, Peter, Kim, Matt, Cooper, Cade, Michalik, Meagan, Siew, Benjamin, Turesson, Elise, Maupin-Furlow, Julie A.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 25-12-2022 Elsevier
Subjects:	Amino acid exchange BASIC BIOLOGICAL SCIENCES Biochemistry & Molecular Biology Biotechnology & Applied Microbiology DNA Method Mutagenesis, Site-Directed Mutation PCR Plasmids - genetics Polymerase Chain Reaction - methods Site-directed mutagenesis Substitution mutation ss Apr rrPCR SSPER PAGE Nvr Method Substitution mutation 6-FAM LB Site-directed mutagenesis Amino acid exchange SDM PCR CFUs
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