Potential Neuroprotective Role of Calretinin-N18 and Calbindin-D28k in the Retina of Adult Zebrafish Exposed to Different Wavelength Lights

• Published in: International journal of molecular sciences Vol. 24; no. 2; p. 1087
• Main Authors: Porcino, Caterina, Briglia, Marilena, Aragona, Marialuisa, Mhalhel, Kamel, Laurà, Rosaria, Levanti, Maria, Abbate, Francesco, Montalbano, Giuseppe, Germanà, Germana, Lauriano, Eugenia Rita, Meduri, Alessandro, Vega, Josè Antonio, Germanà, Antonino, Guerrera, Maria Cristina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06-01-2023; MDPI
• Subjects: Animals; CaBPs; Calbindin 1; Calbindin 2; Calbindin-D28K; Calbindins; Calretinin; Danio rerio; Electronic equipment; Exposure; Homeostasis; Immunofluorescence; Investigations; Laser microscopy; Localization; Neuroprotection; Oxidative stress; Pathogenesis; Photoreceptors; Proteins; Radiation; Retina; Retina - metabolism; S100 Calcium Binding Protein G - metabolism; Visual impairment; Zebrafish; Zebrafish - metabolism; retina; CaBPs; zebrafish
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24021087

The incidence rates of light-induced retinopathies have increased significantly in the last decades because of continuous exposure to light from different electronic devices. Recent studies showed that exposure to blue light had been related to the pathogenesis of light-induced retinopathies. However, the pathophysiological mechanisms underlying changes induced by light exposure are not fully known yet. In the present study, the effects of exposure to light at different wavelengths with emission peaks in the blue light range (400-500 nm) on the localization of Calretinin-N18 (CaR-N18) and Calbindin-D28K (CaB-D28K) in adult zebrafish retina are studied using double immunofluorescence with confocal laser microscopy. CaB-D28K and CaR-N18 are two homologous cytosolic calcium-binding proteins (CaBPs) implicated in essential process regulation in central and peripheral nervous systems. CaB-D28K and CaR-N18 distributions are investigated to elucidate their potential role in maintaining retinal homeostasis under distinct light conditions and darkness. The results showed that light influences CaB-D28K and CaR-N18 distribution in the retina of adult zebrafish, suggesting that these CaBPs could be involved in the pathophysiology of retinal damage induced by the short-wavelength visible light spectrum.