Bioperformance Studies of Biphasic Calcium Phosphate Scaffolds Extracted from Fish Bones Impregnated with Free Curcumin and Complexed with β-Cyclodextrin in Bone Regeneration

• Published in: Biomolecules (Basel, Switzerland) Vol. 12; no. 3; p. 383
• Main Authors: Truite, Cecilia V R, Noronha, Jessica N G, Prado, Gabriela C, Santos, Leonardo N, Palácios, Raquel S, do Nascimento, Adriane, Volnistem, Eduardo A, da Silva Crozatti, Thamara T, Francisco, Carolina P, Sato, Francielle, Weinand, Wilson R, Hernandes, Luzmarina, Matioli, Graciette
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28-02-2022; MDPI
• Subjects: Animals; Apatite; beta-Cyclodextrins - pharmacology; Biological activity; biomaterials; Biomedical materials; biphasic calcium phosphate; Bone biomaterials; Bone growth; Bone healing; Bone Regeneration; Bones; Calcium phosphates; Calvaria; Curcumin; Curcumin - pharmacology; Fish; Fourier transforms; Growth factors; Hydroxyapatites; materials engineering; Morphology; Osteogenesis; Particle size; Physicochemical properties; Porosity; Powder metallurgy; Rats; Regeneration; Tilapia; Tissue engineering; Tissue Scaffolds - chemistry; β-Cyclodextrin; United Kingdom > UK; Japan; Germany; bone regeneration; biomaterials; materials engineering; biphasic calcium phosphate; β-cyclodextrin; curcumin
• DOI: 10.3390/biom12030383
• EISSN: 2218-273X

Fish bones are a natural calcium phosphate (CaP) sources used in biomaterials production for bone regeneration. CaP scaffolds can be enriched with other substances with biological activity to improve bone repair. This study aimed to evaluate the physicochemical properties and bone regeneration potential of biphasic calcium phosphate (BCP) scaffolds impregnated with free curcumin (BCP-CL) or complexed with β-cyclodextrin (BCP-CD) compared to BCP scaffolds. Rietveld's refinement showed that BCP is composed of 57.2% of HAp and 42.8% of β-TCP and the molar ratio of Ca/P corresponds to 1.59. The scaffolds presented porosity (macro and microporosity) of 57.21%. Apatite formation occurred on the BCP, BCP-CL, and BCP-CD surface, in vitro, in SBF. Micro-Raman technique showed a reduction in the dissolution rate of β-TCP in the curcumin-impregnated scaffolds over time, and in vivo studies on critical-size defects, in rat calvaria, had no additional regenerative effect of BCP-CL and BCP-CD scaffolds, compared to BCP scaffolds. Despite this, the study showed that curcumin impregnation in BCP scaffolds prolongs the release of the β-TCP phase, the BCP- phase with the higher osteoinductive potential, representing an advantage in tissue engineering.