Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells

• Published in: Biomolecules (Basel, Switzerland) Vol. 10; no. 1; p. 139
• Main Authors: Kello, Martin, Takac, Peter, Kubatka, Peter, Kuruc, Tomas, Petrova, Klaudia, Mojzis, Jan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14-01-2020; MDPI
• Subjects: AKT protein; Antibodies; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Antioxidants; Apoptosis; Apoptosis - drug effects; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Cancer; Caspase; Cell cycle; Cell death; Chemotherapy; clove buds extract; Cytochrome; Deoxyribonucleic acid; DNA; DNA damage; DNA Damage - drug effects; Ethanol; Experiments; Female; Free radicals; Humans; Immunomodulation; Inflammation; Kinases; MAP kinase; MCF-7 Cells; Mitochondria; Nitrogen; Nitrogen radicals; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation; Phytochemicals; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Radiation therapy; Signal transduction; Spices; stress/survival pathways; Superoxide dismutase; Syzygium - chemistry; Tumors; United Kingdom > UK; United States > US; Germany; apoptosis; breast cancer; clove buds extract; DNA damage; stress/survival pathways; oxidative stress
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom10010139

In recent decades, several spices have been studied for their potential in the prevention and treatment of cancer. It is documented that spices have antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. The main mechanisms of spices action included apoptosis induction, proliferation, migration and invasion of tumour inhibition, and sensitization of tumours to radiotherapy and chemotherapy. In this study, the ability of clove buds extract (CBE) to induce oxidative stress, DNA damage, and stress/survival/apoptotic pathways modulation were analysed in MCF-7 cells. We demonstrated that CBE treatment induced intrinsic caspase-dependent cell death associated with increased oxidative stress mediated by oxygen and nitrogen radicals. We showed also the CBE-mediated release of mitochondrial pro-apoptotic factors, signalling of oxidative stress-mediated DNA damage with modulation of cell antioxidant SOD (superoxide dismutase) system, and modulation activity of the Akt, p38 MAPK, JNK and Erk 1/2 pathways.