BnERF114.A1 , a Rapeseed Gene Encoding APETALA2/ETHYLENE RESPONSE FACTOR, Regulates Plant Architecture through Auxin Accumulation in the Apex in Arabidopsis

• Published in: International journal of molecular sciences Vol. 23; no. 4; p. 2210
• Main Authors: Lyu, Jinyang, Guo, Yuan, Du, Chunlei, Yu, Haibo, Guo, Lijian, Liu, Li, Zhao, Huixian, Wang, Xinfa, Hu, Shengwu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17-02-2022; MDPI
• Subjects: Accumulation; Acetic acid; Agricultural production; Amino Acid Sequence; Apexes; Apical dominance; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Auxins; Biosynthesis; BnERF114.A1; Brassica napus; Brassica napus - genetics; Brassica rapa - genetics; C-Terminus; Cell division; Cell growth; Efflux; Ethylene; Flowers & plants; Gene Expression Regulation, Plant - genetics; Genes; Indoleacetic acid; Indoleacetic Acids - metabolism; Kinases; Leaves; Meristems; Organs; Phylogenetics; plant architecture; Plant Breeding - methods; Plant Leaves - genetics; Primordia; Proteins; Rape plants; Rapeseed; rapeseed (Brassica napus L.); Reproductive organs; seed yield; Seeds - genetics; shoot branching; Signal transduction; Transcription factors; Transcription Factors - genetics; China; shoot branching; rapeseed (Brassica napus L.); BnERF114.A1; plant architecture; seed yield; apical dominance
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23042210

Plant architecture is crucial for rapeseed breeding. Here, we demonstrate the involvement of
, a transcription factor for ETHYLENE RESPONSE FACTOR (ERF), in the regulation of plant architecture in
.
is a member of the ERF family group X-a, encoding a putative 252-amino acid (aa) protein, which harbours the AP2/ERF domain and the conserved CMX-1 motif.
is localised to the nucleus and presents transcriptional activity, with the functional region located at 142-252 aa of the C-terminus. GUS staining revealed high
expression in leaf primordia, shoot apical meristem, leaf marginal meristem, and reproductive organs. Ectopic
expression in
reduced plant height, increased branch and silique number per plant, and improved seed yield per plant. Furthermore, in
,
overexpression inhibited indole-3-acetic acid (IAA) efflux, thus promoting auxin accumulation in the apex and arresting apical dominance. Therefore,
probably plays an important role in auxin-dependent plant architecture regulation.