Analysis of fumonisin contamination and the presence of Fusarium in wheat with kernel black point disease in the United States

• Published in: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Vol. 29; no. 7; pp. 1092 - 1100
• Main Authors: Busman, M, Desjardins, A.E, Proctor, R.H
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Group 01-01-2012; Taylor & Francis; Taylor & Francis Ltd
• Subjects: Biological and medical sciences; black point disease; Cereal and baking product industries; cereals and grain; Chromatography, Liquid; conidia; Food contamination & poisoning; Food industries; Food toxicology; fumonisins; Fumonisins - analysis; Fundamental and applied biological sciences. Psychology; Fungi; Fusarium - isolation & purification; Fusarium proliferatum; greenhouse experimentation; LC/MS; Mass Spectrometry; microbiology; mycotoxins - fumonisins; Plant Diseases - microbiology; risk; Risk assessment; seeds; survey; Toxins; Triticum - microbiology; Triticum aestivum; United States; Wheat; United States; North America; America; United States > US; USA; Disease; mycotoxins; Mycotoxin; microbiology; Liquid chromatography; cereals and grain; Contamination; Fungi; LC/MS; Toxin; Survey; Surveillance; Analysis; fumonisins; Fusarium; Cereal; Fungi Imperfecti; Fumonisin; Wheat
• ISSN: 1944-0057; 1944-0049; 1944-0057
• DOI: 10.1080/19440049.2012.671787

The ability of the fungus Fusarium proliferatum to cause kernel black point disease in wheat was previously established, but natural contamination of black point wheat with both F. proliferatum and fumonisin mycotoxins has not been studied in the United States. Low levels of fumonisins were detected in nine of 43 wheat samples with kernel black point disease that were obtained from across the United States. A subset of samples was contaminated with F. proliferatum as well as with F. fujikuroi, F. nygamai, F. thapsinum and F. verticillioides, species closely related to F. proliferatum and morphologically similar to it in that they produce chains of asexual spores, or conidia. Nevertheless, of conidial chain-forming fusaria isolated from symptomatic wheat, F. proliferatum dominated. In greenhouse tests, isolates of F. proliferatum and the other species recovered from wheat samples were able to cause symptoms of kernel black point and, in some cases, low levels of fumonisin contamination of wheat. These data add to the understanding of the risk of fumonisin contamination of wheat and the potential for Fusarium species to cause kernel black point disease and fumonisin contamination of wheat. Further, the results of this study indicate that while US-grown wheat can sporadically be contaminated by fumonisins, the natural contamination levels seem to be low. The observations made provide evidence that fumonisins are not likely to be a factor contributing to the ability of Fusarium to cause kernel black point disease.