Short-term cooling increases serum triglycerides and small high-density lipoprotein levels in humans

• Published in: Journal of clinical lipidology Vol. 11; no. 4; pp. 920 - 928.e2
• Main Authors: Hoeke, Geerte, Nahon, Kimberly J, Bakker, Leontine E.H, Norkauer, Sabine S.C, Dinnes, Donna L.M, Kockx, Maaike, Lichtenstein, Laeticia, Drettwan, Diana, Reifel-Miller, Anne, Coskun, Tamer, Pagel, Philipp, Romijn, Fred P.H.T.M, Cobbaert, Christa M, Jazet, Ingrid M, Martinez, Laurent O, Kritharides, Leonard, Berbée, Jimmy F.P, Boon, Mariëtte R, Rensen, Patrick C.N
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2017
• Subjects: Adult; ATP Binding Cassette Transporter 1 - metabolism; Biological Transport; Brown adipose tissue; Cardiovascular; Cholesterol; Cholesterol - metabolism; Cold exposure; Cold Temperature; Healthy Volunteers; High-density lipoprotein; Humans; Lipids and lipoprotein metabolism; Lipoproteins, HDL - blood; Lipoproteins, HDL - chemistry; Male; Particle Size; Time Factors; Triglycerides - blood; Young Adult; Brown adipose tissue; Lipids and lipoprotein metabolism; High-density lipoprotein; Cold exposure; Cholesterol
• ISSN: 1933-2874; 1876-4789
• DOI: 10.1016/j.jacl.2017.04.117

Background Cold exposure and β3-adrenergic receptor agonism, which both activate brown adipose tissue, markedly influence lipoprotein metabolism by enhancing lipoprotein lipase–mediated catabolism of triglyceride-rich lipoproteins and increasing plasma high-density lipoprotein (HDL) levels and functionality in mice. However, the effect of short-term cooling on human lipid and lipoprotein metabolism remained largely elusive. Objective The objective was to assess the effect of short-term cooling on the serum lipoprotein profile and HDL functionality in men. Methods Body mass index–matched young, lean men were exposed to a personalized cooling protocol for 2 hours. Before and after cooling, serum samples were collected for analysis of lipids and lipoprotein composition by1 H-nuclear magnetic resonance. Adenosine triphosphate–binding cassette A1 (ABCA1)-mediated cholesterol efflux capacity of HDL was measured using [3 H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. Results Short-term cooling increased serum levels of free fatty acids, triglycerides, and cholesterol. Cooling increased the concentration of large very low–density lipoprotein (VLDL) particles accompanied by increased mean size of VLDL particles. In addition, cooling enhanced the concentration of small LDL and small HDL particles as well as the cholesterol levels within these particles. The increase in small HDL was accompanied by increased ABCA1-dependent cholesterol efflux in vitro. Conclusions Our data show that short-term cooling increases the concentration of large VLDL particles and increases the generation of small LDL and HDL particles. We interpret that cooling increases VLDL production and turnover, which results in formation of surface remnants that form small HDL particles that attract cellular cholesterol.