Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association

• Published in: Plant science (Limerick) Vol. 181; no. 3; pp. 242 - 248
• Main Authors: Cho, Kyoungwon, Han, Younsoo, Woo, Je Chang, Baudisch, Bianca, Klösgen, Ralf Bernd, Oh, Seunghan, Han, Jihoon, Han, Oksoo
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01-09-2011; [Ireland]: Elsevier Science Ireland Ltd; Elsevier
• Subjects: Agronomy. Soil science and plant productions; binding sites; Biological and medical sciences; Calcium; Calcium - metabolism; Calcium-binding protein; Carrier Proteins; Chloroplasts; Chloroplasts - chemistry; Chloroplasts - enzymology; Chloroplasts - metabolism; corn; Cytoplasm; Cytoplasm - chemistry; Cytoplasm - enzymology; Cytoplasm - metabolism; Dual positional specificity; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genes, Plant; Genetic engineering applications; Genetics and breeding of economic plants; in vitro studies; Lipoxygenase; Lipoxygenase - chemistry; Lipoxygenase - genetics; Lipoxygenase - metabolism; Maize; Oryza - enzymology; Oryza - genetics; Oryza - metabolism; Oryza sativa; Plant breeding: fundamental aspects and methodology; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Protein structure; Protein Transport; Rice; Secondary structure; Transgenic plants; Translocation; Zea mays; Zea mays - enzymology; Zea mays - genetics; Zea mays - metabolism; Dual positional specificity; Translocation; Maize; Calcium; Lipoxygenase; Rice; Monocotyledones; Zea mays; Enzyme; Plant biology; Cereal crop; Oryza; Gramineae; Angiospermae; Membrane; Herbaceous plant; Spermatophyta; Transgenic plant; Oxidoreductases
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2011.05.016

The dual positional maize lipoxygenase-1 was introduced into rice and T2 transgenic plants were produced. Cellular location of maize lipoxygenase-1 in transgenic rice and effects of calcium ion on membrane association in vitro were analyzed. Localization study by confocal microscopic analysis indicated that the maize lipoxygenase-1 was localized in cytoplasm. Sucrose-density fractionation experiment and in vitro protein transport to chloroplast showed that the maize lipoxygenase-1 can be associated with chloroplast. Secondary structure alignment revealed putative calcium binding sites in the PLAT domain of maize lipoxygenase-1 and the association of the maize lipoxygenase-1 with membranes was mediated by calcium ion in vitro. Our results provide evidences for calcium-mediated translocation of dual positional LOX without chloroplast targeting sequence from cytoplasm to chloroplast in plants for the first time.