Alginate-coated chitosan nanogels differentially modulate class-A and class-B CpG-ODN targeting of dendritic cells and intracellular delivery

Abstract CpG-oligodeoxynucleotides (CpG-ODNs) interact with dendritic cells (DCs), but evidence is less clear for CpG-ODN admixed with or incorporated into vaccine delivery vehicles. We loaded alginate-coated chitosan-nanogels (Ng) with class-A or class-B CpG-ODN, and compared with the same CpG-ODNs...

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Bibliographic Details
Published in:	Nanomedicine Vol. 10; no. 8; pp. 1739 - 1749
Main Authors:	Démoulins, Thomas, PhD, Milona, Panagiota, PhD, McCullough, Kenneth C., PhD
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-11-2014
Subjects:	Alginates - chemistry Animals Blood dendritic cell targeting Cells, Cultured Chitosan - chemistry CpG-ODN Dendritic Cells - metabolism Flow Cytometry Glucuronic Acid - chemistry Hexuronic Acids - chemistry Humans Internal Medicine Intracellular delivery Microscopy, Confocal Nanoparticles Oligodeoxyribonucleotides - chemistry Swine fluorescence-activated cell sorter plasmacytoid dendritic cells PE, phycoerythrin DLS FACS Nanoparticles DMEM MFI FITC dendritic cells DCs TPP pDCs Toll-like receptors Ng PAMPs sodium triphosphate, pentabasic TLR fluorescein isothiocyanate peripheral blood mononuclear cells mean fluorescence intensity Blood dendritic cell targeting Intracellular delivery PBMCs Dulbecco's modified Eagle's medium nanogel pathogen-associated molecular patterns conventional (also known as classical) dendritic cells CpG oligodeoxynucleotide enzyme-linked immunosorbent assay CpG-ODN Dynamic Light Scattering cDCs ELISA
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