Excision Repair of Benzo[a]pyrene-deoxyguanosine Adducts in baby Hamster Kidney 21/C13 Cells and in Secondary Mouse Embryo Fibroblasts C57BL/6J

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 74; no. 3; pp. 979 - 983
• Main Authors: Shinohara, Kunio, Cerutti, Peter A.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-03-1977; National Acad Sciences
• Subjects: Adducts; Benzopyrenes - metabolism; Borates; Cell Line; Cell lines; Deoxyribonucleosides; DNA; DNA damage; DNA Repair; Embryonic cells; Guanosine - analogs & derivatives; Guanosine - metabolism; Kidney cells; Kinetics; Lesions; Radioactive decay; Radiocarbon
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.74.3.979

The formation and excision of benzo[a]pyrene-deoxyguanosine adducts in metabolizing baby hamster kidney cells (21/C13) and secondary mouse embryo fibroblasts (C57BL/6J) was investigated. Both diastereomeric adducts, N2-(10-{7β ,8α ,9α -trihydroxy- 7, 8, 9, 10-tetrahydrobenzo[a]-pyrene}yl)deoxyguanosine and N2-(10-{7β ,8α ,9β -trihydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene}yl)deoxyguanosine, were detected in both cell lines and both cell lines were capable of excising these lesions, albeit with low efficiency.