Fine-mapping and candidate gene analysis for the foxglove aphid resistance gene Raso2 from wild soybean PI 366121

• Published in: Theoretical and applied genetics Vol. 134; no. 8; pp. 2687 - 2698
• Main Authors: Kim, Ki-Seung, Kim, Ji-Min, Jung, Jiyeong, Shin, Ilseob, Park, Sumin, Lee, Ju Seok, Jeong, Soon-Chun, Lee, Jeong-Dong, Jung, Jin Kyo, Ha, Bo-Keun, Kang, Sungtaeg
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2021; Springer; Springer Nature B.V
• Subjects: Agriculture; Analysis; Animals; Antibiosis; Aphididae; Aphids - physiology; Aulacorthum solani; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome 7; Chromosome Mapping - methods; Chromosomes, Plant - genetics; Cultivars; Disease resistance; Disease Resistance - genetics; Disease Resistance - immunology; Gene Expression Regulation, Plant; Gene mapping; Genes; Genomes; Genomics; Glycine max - genetics; Glycine max - growth & development; Glycine max - parasitology; Glycine soja; Heredity; Inbreeding; Insect pests; Life Sciences; Marker-assisted selection; Original Article; Phenotype; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - parasitology; Plant Genetics and Genomics; Plant Proteins - genetics; Plant Proteins - metabolism; Polymorphism, Single Nucleotide; Single-nucleotide polymorphism; Soybean; Soybeans; South Korea; Japan
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-021-03853-8

Key message
The foxglove aphid resistance gene
Raso2
from PI 366121 was fine-mapped to 77 Kb region, and one candidate gene was identified.
The foxglove aphid (FA:
Aulacorthum solani
Kaltenbach) is an important insect pest that causes serious yield losses in soybean. The FA resistance gene
Raso2
from wild soybean PI 366121 was previously mapped to a 13 cM interval on soybean chromosome 7. However, fine-mapping of
Raso2
was needed to improve the effectiveness of marker-assisted selection (MAS) and to eventually clone it. The objectives of this study were to fine-map
Raso2
from PI 366121 using Axiom
®
180 K SoyaSNP array, to confirm the resistance and inheritance of
Raso2
in a different background, and to identify candidate gene(s). The 105 F
4:8
recombinant inbred lines were used to fine-map the gene and to test antibiosis and antixenosis of
Raso2
to FA. These efforts resulted in the mapping of
Raso2
on 1 cM interval which corresponds to 77 Kb containing eight annotated genes based on the Williams 82 reference genome assembly (Wm82.a2.v1). Interestingly, all nonsynonymous substitutions were in
Glyma.07g077700
which encodes the disease resistance protein containing LRR domain and expression of the gene in PI 366121 was significantly higher than that in Williams 82. In addition, distinct SNPs within
Glyma.07g077700
that can distinguish PI 366121 and diverse FA-susceptible soybeans were identified. We also confirmed that
Raso2
presented the resistance to FA and the Mendelian inheritance for single dominant gene in a different background. The results of this study would provide fundamental information on MAS for development of FA-resistant cultivars as well as functional study and cloning of the candidate gene in soybean.