Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors

Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro sponta...

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Bibliographic Details
Published in:	Stem cell research Vol. 21; no. C; pp. 16 - 18
Main Authors:	Táncos, Zsuzsanna, Nemes, Csilla, Varga, Eszter, Bock, István, Rungarunlert, Sasitorn, Tharasanit, Theerawat, Techakumphu, Mongkol, Kobolák, Julianna, Dinnyés, András
Format:	Journal Article
Language:	English
Published:	England Elsevier 01-05-2017
Subjects:	Animals Cell Line Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Lentivirus Rabbits Transcription Factors - biosynthesis Transcription Factors - genetics Transduction, Genetic
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Summary:	Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. Furthermore, the spontaneous differentiation potential of the iPSC was also tested in vivo by teratoma assay. The iPSC line showed normal karyotype. The advantages of using RbiPSC are the easy access to primary material and the possibility to study the efficacy and safety of the iPSC-based therapies on a non-rodent animal model.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2017.03.010