Expression of the aryl hydrocarbon receptor is not required for the proliferation, migration, invasion, or estrogen-dependent tumorigenesis of MCF-7 breast cancer cells

• Published in: Molecular carcinogenesis Vol. 52; no. 7; pp. 544 - 554
• Main Authors: Spink, Barbara C., Bennett, James A., Lostritto, Nicole, Cole, Jacquelyn R., Spink, David C.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-07-2013; Wiley Subscription Services, Inc
• Subjects: Animals; Apoptosis - drug effects; Aryl Hydrocarbon Hydroxylases - genetics; Aryl Hydrocarbon Hydroxylases - metabolism; aryl hydrocarbon receptor; Blotting, Western; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell adhesion & migration; Cell Adhesion - drug effects; Cell Cycle - drug effects; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Transformation, Neoplastic - metabolism; Cell Transformation, Neoplastic - pathology; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P-450 CYP1B1; Estrogens; Estrogens - pharmacology; Female; Humans; Immunoenzyme Techniques; MCF-7 xenograft; Mice; Mice, SCID; Neovascularization, Pathologic; Oncology; Receptors, Aryl Hydrocarbon - genetics; Receptors, Aryl Hydrocarbon - metabolism; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Tumor Cells, Cultured; Tumors
• ISSN: 0899-1987; 1098-2744
• DOI: 10.1002/mc.21889

The AhR was initially identified as a ligand‐activated transcription factor mediating effects of chlorinated dioxins and polycyclic aromatic hydrocarbons on cytochrome P450 1 (CYP1) expression. Recently, evidence supporting involvement of the AhR in cell‐cycle regulation and tumorigenesis has been presented. To further define the roles of the AhR in cancer, we investigated the effects of AhR expression on cell proliferation, migration, invasion, and tumorigenesis of MCF‐7 human breast cancer cells. In these studies, the properties of MCF‐7 cells were compared with those of two MCF‐7‐derived sublines: AHR100, which express minimal AhR, and AhRexp, which overexpress AhR. Quantitative PCR, Western immunoblots, 17β‐estradiol (E2) metabolism assays, and ethoxyresorufin O‐deethylase assays showed the lack of AhR expression and AhR‐regulated CYP1 expression in AHR100 cells, and enhanced AhR and CYP1 expression in AhRexp cells. In the presence of 1 nM E2, rates of cell proliferation of the three cell lines showed an inverse correlation with the levels of AhR mRNA. In comparison with MCF‐7 and AhRexp cells, AHR100 cells produced more colonies in soft agar and showed enhanced migration and invasion in chamber assays with E2 as the chemoattractant. Despite the lack of significant AhR expression, AHR100 cells retained the ability to form tumors in severe combined immunodeficient mice when supplemented with E2, producing mean tumor volumes comparable to those observed with MCF‐7 cells. These studies indicate that, while CYP1 expression and inducibility are highly dependent on AhR expression, the proliferation, invasion, migration, anchorage‐independent growth, and estrogen‐stimulated tumor formation of MCF‐7 cells do not require the AhR. © 2012 Wiley Periodicals, Inc.