The Toxicity of a Lipid Transfer Protein (Cc-LTP1) from Coffea canephora Seeds on the Larval Development of Callosobruchus maculatus (Coleoptera: Bruchidae)

• Published in: Protein Journal Vol. 33; no. 5; pp. 422 - 431
• Main Authors: Zottich, Umberto, Da Cunha, Maura, Dias, Germana B., Rabelo, Guilherme R., Oliveira, Antonia Elenir A., Carvalho, André O., Fernandes, Kátia Valevski S., do Nascimento, Viviane V., Gomes, Valdirene M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-10-2014; Springer Nature B.V
• Subjects: Animal Anatomy; Animals; Antimicrobial agents; Biochemistry; Bioorganic Chemistry; Bruchidae; Callosobruchus maculatus; Carrier Proteins - toxicity; Chemistry; Chemistry and Materials Science; Coffea; Coffea - chemistry; Coleoptera; Coleoptera - drug effects; Coleoptera - growth & development; Female; Histology; Larva - drug effects; Larva - growth & development; Larvae; Larval development; Lipids; Mitochondria; Morphology; Organic Chemistry; Peptides; Pests; Proteins; Seeds - chemistry; Vigna unguiculata; Lipid transfer protein; Defense protein
• ISSN: 1572-3887; 1573-4943; 1875-8355
• DOI: 10.1007/s10930-014-9575-9

In this work, we analyzed the effects of coffee seed proteins, especially
Cc
-LTP
1
on the larval development of
Callosobruchus maculatus
(F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of
Vigna unguiculata
(L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from
Coffea canephora
seeds, resulted in the reduction of oviposition and caused an inhibition of
C. maculatus
larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified
Cc
-LTP
1
, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae.
Cc
-LTP
1
was also able to inhibit the
C. maculatus
gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the
C. maculatus
larvae.
Cc
-LTP
1
has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that
Cc
-LTP
1
has insecticidal actions toward
C. maculatus
and exerts anti-nutritional effects with direct actions on intestinal tissues.