Altered EZH2 splicing and expression is associated with impaired histone H3 lysine 27 tri-Methylation in myelodysplastic syndrome

• Published in: Leukemia research Vol. 63; pp. 90 - 97
• Main Authors: Shirahata-Adachi, Mizuho, Iriyama, Chisako, Tomita, Akihiro, Suzuki, Yasuhiro, Shimada, Kazuyuki, Kiyoi, Hitoshi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-12-2017
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Alternative Splicing; Biomarkers, Tumor - genetics; DNA Methylation; Enhancer of Zeste Homolog 2 Protein - genetics; Enhancer of Zeste Homolog 2 Protein - metabolism; Epigenesis, Genetic; EZH2; Female; Follow-Up Studies; H3K27 methyltransferase; Histones - genetics; Humans; Lysine - genetics; Male; Middle Aged; Mutation; Myelodysplastic syndromes; Myelodysplastic Syndromes - genetics; Myelodysplastic Syndromes - metabolism; Myelodysplastic Syndromes - pathology; Prognosis; Tumor Cells, Cultured; Young Adult; H3K27 methyltransferase; Myelodysplastic syndromes; EZH2
• DOI: 10.1016/j.leukres.2017.10.015
• EISSN: 1873-5835
• ISSN: 0145-2126

•EZH2 expression profiles in bone marrow cells from MDS patients are characterized.•EZH2 expression levels and transcript sizes varied considerably between patients.•Altered splicing mainly induces multiple EZH2 transcripts.•Frameshift mutations resulted in truncated EZH2 protein translation.•Truncated proteins impair endogenous function and contribute to MDS pathogenesis.
EZH2 (enhancer of zeste homolog 2) is a histone H3K27 methyltransferase involved in the pathogenesis of various hematological malignancies. In myelodysplastic syndromes (MDS), loss of function of EZH2 is known to contribute to pathogenesis, however the pattern of EZH2 mRNA and protein expression in MDS has not been extensively characterized.
A total of 26 patients diagnosed with MDS were analyzed in this study. The relationship between EZH2 expression in patient bone marrow samples, evaluated by RT-PCR and immunoblotting, and patient characteristics were analyzed. The function of truncated EZH2 proteins was examined in vitro.
EZH2 expression levels and transcript sizes varied considerably between patients, but there was no relationship with the percentage blast component of patient samples. Cloning and sequencing of amplified RT-PCR fragments demonstrated that patients expressed multiple EZH2 transcripts containing insertions or deletions, with or without frameshift, mainly induced by altered splicing. All identified frameshift mutations were found to be 5′ to the functional SET domain, and resulted in truncated protein translation. Altered patterns of EZH2 expression was observed in patients with or without alterations in genes involved with RNA splicing, SRSF2, U2AF1 and SF3B1. Functional analysis in vitro revealed that C-terminally truncated EZH2, lacking the SET domain, may impair the methyltransferase function of wild-type EZH2 in a dominant negative fashion.
Our findings suggest that the loss of function of EZH2 induced by aberrant splicing, and/or EZH2 mutations resulting in the production of C-terminally truncated proteins, may be involved in MDS pathogenesis.