Altered Fine Structures of Corneal and Skeletal Keratan Sulfate and Chondroitin/Dermatan Sulfate in Macular Corneal Dystrophy

• Published in: The Journal of biological chemistry Vol. 276; no. 43; pp. 39788 - 39796
• Main Authors: Plaas, Anna H., West, Leigh A., Thonar, Eugene J.A., Karcioglu, Zeynel A., Smith, Clayton J., Klintworth, Gordon K., Hascall, Vincent C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26-10-2001; American Society for Biochemistry and Molecular Biology
• Subjects: Acetylglucosamine - analysis; Adult; Aged; Carbohydrate Sequence; Cartilage - chemistry; Chondroitin Sulfates - chemistry; Cornea - chemistry; Corneal Dystrophies, Hereditary - pathology; Dermatan Sulfate - chemistry; Fucose - analysis; Glycopeptides - chemistry; Glycosaminoglycans - chemistry; Humans; Keratan Sulfate - chemistry; Middle Aged; Molecular Sequence Data; Oligosaccharides - chemistry
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M103227200

The content and fine structure of keratan and chondroitin/dermatan sulfate in normal human corneas and corneas affected by macular corneal dystrophies (MCD) types I and II were examined by fluorophore-assisted carbohydrate electrophoresis. Normal tissues (n = 11) contained 15 µg of keratan sulfate and 8 µg of chondroitin/dermatan sulfate per mg dry weight. Keratan sulfates consisted of ∼4% unsulfated, 42% monosulfated, and 54% disulfated disaccharides with number of average chain lengths of ∼14 disaccharides. Chondroitin/dermatan sulfates were significantly longer, ∼40 disaccharides per chain, and consisted of ∼64% unsulfated, 28% 4-sulfated, and 8% 6-sulfated disaccharides. The fine structural parameters were altered in all diseased tissues. Keratan sulfate chain size was reduced to 3–4 disaccharides; chain sulfation was absent in MCD type I corneas and cartilages, and sulfation of both GlcNAc and Gal was significantly reduced in MCD type II. Chondroitin/dermatan sulfate chain sizes were also decreased in all diseased corneas to ∼15 disaccharides, and the contents of 4- and 6-sulfated disaccharides were proportionally increased. Tissue concentrations (nanomole of chains per mg dry weight) of all glycosaminoglycan types were affected in the disease types. Keratan sulfate chain concentrations were reduced by ∼24 and ∼75% in type I corneas and cartilages, respectively, and by ∼50% in type II corneas. Conversely, chondroitin/dermatan sulfate chain concentrations were increased by 60–70% in types I and II corneas. Such changes imply a modified tissue content of individual proteoglycans and/or an altered efficiency of chain substitution on the core proteins. Together with the finding that hyaluronan, not normally present in healthy adult corneas, was also detected in both disease subtypes, the data support the conclusion that a wide range of keratocyte-specific proteoglycan and glycosaminoglycan remodeling processes are activated during degeneration of the stromal matrix in the macular corneal dystrophies.