Utilization of chicory roots for microbial endoinulinase production

• Published in: Letters in applied microbiology Vol. 33; no. 3; pp. 183 - 187
• Main Authors: Park, J.P., Yun, J.W.
• Format: Journal Article
• Language: English
• Published: Oxford UK Blackwell Science Ltd 01-09-2001; Blackwell Science
• Subjects: Biological and medical sciences; Biotechnology; Cichorium intybus - metabolism; Culture Media; endoinulase; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; Glycoside Hydrolases - biosynthesis; Hydrogen-Ion Concentration; Inulin - metabolism; Methods. Procedures. Technologies; Plant Roots - metabolism; Production of selected enzymes; Pseudomonas; Pseudomonas - enzymology; Pseudomonas - growth & development; Temperature; Xanthomonas; Xanthomonas - enzymology; Xanthomonas - growth & development; Pseudomonadales; Cichorium intybus; Root; Enzyme; Compositae; Pseudomonas; Xanthomonas; Dicotyledones; Glycosidases; Inulinase; Angiospermae; Production; Microorganism culture; Bacteria; Hydrolases; Pseudomonadaceae; Spermatophyta; O-Glycosidases; Chicory extract
• ISSN: 0266-8254; 1472-765X; 1365-2673
• DOI: 10.1046/j.1472-765x.2001.00977.x

Aims: The optimal culture conditions for endoinulinase production using chicory roots were studied in shake‐flask culture.
Methods and Results: Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml–1) than with Pseudomonas sp. (3 U ml–1). Optimized culture conditions of Xanthomonas sp. for endoinulinase production in flask culture were: chicory powder, 5 g l–1; temperature, 37°C; pH, 7·0; agitation speed, 100 rev min–1.
Conclusions: Maximum bacterial growth and enzyme production were 6·2 g l–1 and 20 U ml–1 under optimal conditions, respectively.
Significance and Impact of the Study: Chicory roots could be used as a fermentation substrate for the production of enndoinulinase.