Insights into the mechanism of diurnal variations in methane emission from the stem surfaces of Alnus japonica

• Published in: The New phytologist Vol. 235; no. 5; pp. 1757 - 1766
• Main Authors: Takahashi, Kenshi, Sakabe, Ayaka, Azuma, Wakana A., Itoh, Masayuki, Imai, Tomoya, Matsumura, Yasuki, Tateishi, Makiko, Kosugi, Yoshiko
• Format: Journal Article
• Language: English
• Published: Lancaster Wiley Subscription Services, Inc 01-09-2022
• Subjects: Alnus japonica; Anatomy; Diurnal variations; Electron microscopy; Emission analysis; Emissions; fine‐roots; Fluctuations; Methane; methane flux; optical and cryo‐SEM images; Passageways; Pollution monitoring; Roots; sap flux; Scanning electron microscopy; stem; Stems; Transport; Xylem
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.18283

Summary
Recent studies have suggested that in certain environments, tree stems emit methane (CH4). This study explored the mechanism of CH4 emission from the stem surfaces of Alnus japonica in a riparian wetland. Stem CH4 emission rates and sap flux were monitored year‐round, and fine‐root anatomy was investigated.
CH4 emission rates were estimated using a closed‐chamber method. Sap flux was measured using Granier‐type thermal dissipation probes. Root anatomy was studied using both optical and cryo‐scanning electron microscopy.
CH4 emissions during the leafy season exhibited a diurnally changing component superimposed upon an underlying continuum in which the diurnal variation was in phase with sap flux. We propose a model in which stem CH4 emission involves at least two processes: a sap flux‐dependent component responsible for the diurnal changes, and a sap flux‐independent component responsible for the background continuum. The contribution ratios of the two processes are season‐dependent.
The background continuum possibly resulted from the diffusive transport of gaseous CH4 from the roots to the upper trunk. Root anatomy analysis indicated that the intercellular space of the cortex and empty xylem cells in fine roots could serve as a passageway for transport of gaseous CH4.