Combined molecular MRI and immuno-spin-trapping for in vivo detection of free radicals in orthotopic mouse GL261 gliomas

Free radicals play a major role in gliomas. By combining immuno-spin-trapping (IST) and molecular magnetic resonance imaging (mMRI), in vivo levels of free radicals were detected within mice bearing orthotopic GL261 gliomas. The nitrone spin trap DMPO (5,5-dimethyl pyrroline N-oxide) was administere...

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Bibliographic Details
Published in:	Biochimica et biophysica acta Vol. 1832; no. 12; pp. 2153 - 2161
Main Authors:	Towner, Rheal A., Smith, Nataliya, Saunders, Debra, De Souza, Patricia Coutinho, Henry, Leah, Lupu, Florea, Silasi-Mansat, Robert, Ehrenshaft, Marilyn, Mason, Ronald P., Gomez-Mejiba, Sandra E., Ramirez, Dario C.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2013
Subjects:	Albumins Animals Brain Neoplasms - diagnostic imaging Brain Neoplasms - metabolism Brain Neoplasms - pathology Contrast Media Cyclic N-Oxides - immunology Disease Models, Animal Free radical Free Radicals - analysis Free Radicals - isolation & purification Gadolinium DTPA Glioma Glioma - diagnostic imaging Glioma - metabolism Glioma - pathology Immuno-spin-trapping Immunoglobulin G - pharmacology In vivo Magnetic Resonance Imaging Mice Mice, Inbred C57BL Molecular magnetic resonance imaging Mouse Nitrogen Oxides - metabolism Oxidation-Reduction Radiography Spin Labels - chemical synthesis Spin Trapping Tumor Cells, Cultured Tyrosine - analogs & derivatives Tyrosine - metabolism IgG contrast agent Immuno-spin-trapping EDC IHC MRI mMRI HN Gd-DTPA IgG ROS/RNS MDA Free radical BSA NHS PBS ROIs DMPO IST Anti-DMPO probe TE In vivo Glioma Mouse 3-NT Molecular magnetic resonance imaging TR magnetic resonance imaging reactive oxygen (nitrogen) species malondialedhyde IgG-albumin–Gd-DTPA–biotin echo time 3-nitrotyrosine 5,5 dimethyl-1-pyrroline-N-oxide immunohistochemistry gadolinium-diethylene triamine penta acetic acid immunoglobulin G repetition time regions of interest N-succinimidyl 3-(2-pyridyldithio)-propionate phosphate buffer saline 4-hydroxynonenal anti-DMPO antibody–albumin–Gd-DTPA–biotin N-succinimidyl-S-acetylthioacetate molecular MRI bovine serum albumin
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